-Detailed Information [JCRB9065]-

JCRB9065 G-401

Cell information

Important Notice(s)

The origin of G-401.

[The origin of G-401 cell line.]

G-401 cell line was originated from the tomor arose at kidney, and the patient's tumor was diagnosed (classified) as Wilms' tumor.

Later study, however, revealed that G-401 heterotransplant were consistent with a rhabdoid phenotype rather than that of a Wilms' tumor. This was also supported by mRNA expression.
Am J Pathol. 1993 Feb; 142(2): 375–380. 

Therefore the G-401 seemed to be originated from rhabdoid tumor of kidney but not from Wilms' tumor.


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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB9065	Cell Name	G-401
Profile	Rhabdoid tumor of kidney (formerly classified as Wilms' tumor)	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	kidney
Case history	Originally the tumor arose in the patient was classified as Wilms' tumor, but later study on G-401 revealed that the tumor would be rhabdoid tumor of kidney.	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Rhabdoid tumor (formerly described as Wilms' tumor)
Classify	tumor	Established by	Peebles,P.T.
Registered by		Regulation for Distribution
Comment	Prepared from CRL1441.	Year	1987
Medium	McCoy's 5a + FCS 10%	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Cell Number on Passage	split ratio=1/2 - 1/6	Race
CO2 Conc.	5 %	Tissue Sampling	kidney
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0270
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	907299

Reference
Pubmed id:8382007	The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Garvin AJ,Re GG,Tarnowski BI,Hazen-Martin DJ,Sens DA Am J Pathol. 1993 Feb;142(2):375-80

Images

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LOT Information

060195
F00991
020889
06272014

Cell No.	JCRB9065	Cell Name	G-401
LOT No.	060195	Lot Specification	distribution
Medium	MaCoy 5A Iwakata and Grace modification with L-glutamine(ICN) and 10% heat inactivated FBS.	Temperature	37 C
Cell Density at Seeding	3.1x10^6 cells/10cm dish	Methods for Passages	Wash cells with PBS(-) twice, treated with 0.25% trypsin and 0.02% EDTA for 3 min. at room temp.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.1x10^6
Viability at cell freezing (%)	95.0	Antibiotics Used	free
Passage Number	P53	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9065	Cell Name
LOT No.	F00991	Lot Specification	distribution
Medium	McCoy's 5A with 10% FCS (BioWhittaker lot 3M0581)	Temperature	37
Cell Density at Seeding	1/2-1/6	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	3.1 x 10^6
Viability at cell freezing (%)	82.0	Antibiotics Used	free
Passage Number	P54	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human by isoenzymology(NP,G6PD,AST)
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9065	Cell Name	G-401
LOT No.	020889	Lot Specification	distribution
Medium	McCoy's 5a + FCS 10%	Temperature	37 C
Cell Density at Seeding	1/2 - 1/6	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	8.0x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P53	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)	D5S818:13 D13S317:9,14 D7S820:11,14 D16S539:12 VWA:16 TH01:8,10 AM:XY TPOX:8,11 CSF1PO:11,13
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB9065	Cell Name	G-401
LOT No.	06272014	Lot Specification	distribution
Medium	McCoy's 5a medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	2.9 - 3.4 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	D.T. = approx. 42 hrs	Cell Number in Vial (cells/1ml)	5.0 x 10^6
Viability at cell freezing (%)	93.2	Antibiotics Used	free
Passage Number	P54	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:13 D13S317:9,14 D7S820:11,14 D16S539:12 VWA:16 TH01:8,9.3 AM:X,Y TPOX:8,11 CSF1PO:11,13
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - McCoy's 5a	CO2 Conc.	5%
Viability immediately after thawing (%)	93.2	Additional information