JCRB9065 G-401

Cell information

Important Notice(s)

The origin of G-401.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9065 Cell Name   G-401
Profile   Rhabdoid tumor of kidney (formerly classified as Wilms' tumor) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   kidney
Case history   Originally the tumor arose in the patient was classified as Wilms' tumor, but later study on G-401 revealed that the tumor would be rhabdoid tumor of kidney. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Rhabdoid tumor (formerly described as Wilms' tumor)
Classify   tumor Established by   Peebles,P.T.
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1441. Year   1987
Medium   McCoy's 5a + FCS 10% Methods for Passages   0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0270
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907299
Reference
Pubmed id:8382007 The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney.
Garvin AJ,Re GG,Tarnowski BI,Hazen-Martin DJ,Sens DA
Am J Pathol. 1993 Feb;142(2):375-80

Images
Movies



LOT Information

Cell No.   JCRB9065 Cell Name   G-401
LOT No.   060195 Lot Specification   distribution
Medium   MaCoy 5A Iwakata and Grace modification with L-glutamine(ICN) and 10% heat inactivated FBS. Temperature   37 C
Cell Density at Seeding   3.1x10^6 cells/10cm dish Methods for Passages   Wash cells with PBS(-) twice, treated with 0.25% trypsin and 0.02% EDTA for 3 min. at room temp.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.1x10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P53 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9065 Cell Name   
LOT No.   F00991 Lot Specification   distribution
Medium   McCoy's 5A with 10% FCS (BioWhittaker lot 3M0581) Temperature   37
Cell Density at Seeding   1/2-1/6 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by isoenzymology(NP,G6PD,AST)
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9065 Cell Name   G-401
LOT No.   020889 Lot Specification   distribution
Medium   McCoy's 5a + FCS 10% Temperature   37 C
Cell Density at Seeding   1/2 - 1/6 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P53 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:9,14
D7S820:11,14
D16S539:12
VWA:16
TH01:8,10
AM:XY
TPOX:8,11
CSF1PO:11,13
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9065 Cell Name   G-401
LOT No.   06272014 Lot Specification   distribution
Medium   McCoy's 5a medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.9 - 3.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   D.T. = approx. 42 hrs Cell Number in Vial (cells/1ml)   5.0 x 10^6
Viability at cell freezing (%)   93.2 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:9,14
D7S820:11,14
D16S539:12
VWA:16
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:11,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - McCoy's 5a CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93.2
Additional information   
CLOSE