JCRB9066 Chang Liver
Cell information
Important Notice(s)JCRB9066 Chang Liver is HeLa-derivative / Chang Liver細胞のHeLa細胞の汚染
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9066 | Cell Name | Chang Liver |
|---|---|---|---|
| Profile | The Chang Liver cell line has been identified historically as a subline of the HeLa. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | HeLa-contaminant |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | liver, HeLa markers | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | This cell line was reported as a liver cell line but later this cell line was identified as a HeLa cell. | |
| Classify | HeLa | Established by | Chang,R.S. |
| Registered by | Regulation for Distribution | Following rules from ATCC | |
| Comment | Prepared from CCL 13. | Year | 1987 |
| Medium | Basal medium Eagle with 10% calf serum. | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin. |
| Cell Number on Passage | split ratio=1/4 - 1/8 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | (liver) |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | + | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1701344 | Production of interleukin 6 from human liver cell lines: production of interleukin 6 is not concurrent with the production of alpha-fetoprotein. Matsuguchi T,Okamura S,Kawasaki C,Niho Y Cancer Res. 1990 Dec 1;50(23):7457-9 |
| Pubmed id:856839 | Expression of liver and placental alkaline phosphatases in Chang liver cells. Ludueña MA,Iverson GM,Sussman HH J Cell Physiol. 1977 Apr;91(1):119-29 |
| Pubmed id:14185313 | ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS. LEDLEY RS Science. 1964 Oct 9;146(3641):241-3 |
| Pubmed id:14477443 | Clonal variation and interaction of cells with viruses. MURPHY WH,LANDAU BJ Natl Cancer Inst Monogr. 1962 Apr;7():249-71 |
| Pubmed id:13237268 | Continuous subcultivation of epithelial-like cells from normal human tissues. CHANG RS Proc Soc Exp Biol Med. 1954 Nov;87(2):440-3 |
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LOT Information
| Cell No. | JCRB9066 | Cell Name | |
|---|---|---|---|
| LOT No. | 022499 | Lot Specification | distribution |
| Medium | Basal medium Eagle + 10% CS (Biowhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 3.0 x 10^4 cells/ml | Methods for Passages | 0.25% tyrpsin and 0.02% EDTA |
| Doubling Time | ca 36 hrs | Cell Number in Vial (cells/1ml) | 2.0 x 10^6 |
| Viability at cell freezing (%) | 95% | Antibiotics Used | free |
| Passage Number | P261 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD(type A), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9066 | Cell Name | Chang Liver |
|---|---|---|---|
| LOT No. | 072092 | Lot Specification | distribution |
| Medium | BME(lot.062992) + 10% CS (lot.Cell Culture Laboratories, 00021-07) | Temperature | 37 C |
| Cell Density at Seeding | 8.3x10^5 | Methods for Passages | Monolayers are washed once with PBS(-) and treatedwith 0.25% trypsin-0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.8x10^6 |
| Viability at cell freezing (%) | 94.9 | Antibiotics Used | free |
| Passage Number | P261 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LD,NP |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9066 | Cell Name | Chang Liver |
|---|---|---|---|
| LOT No. | 012089 | Lot Specification | distribution |
| Medium | BME + CS 10% | Temperature | 37 C |
| Cell Density at Seeding | 1/4 - 1/8 | Methods for Passages | 0.02% EDTA and 0.25% trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P261 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |