JCRB1224 UC704

Cell information

Important Notice(s)

不死化していないヒト体性幹細胞の分譲について(倫理的な取り扱い)

On the distribution of human non-immortalized somatic stem cells to oversea users (ethical ragulation).

M061101 medium

Cell type:primary mesenchymal stem cell (View Pricing Information)

JCRB No.   JCRB1224 Cell Name   UC704
Profile   Human primary umbilical cord-derived cells with fibroblastic morphology. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   umbilical cord
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   primary Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2007
Medium   M061101 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-fifth of harvested cells.
Cell Number on Passage   2 x 10^5 cells/10cm dish Race   Japanese
CO2 Conc.   5% Tissue Sampling   umbilical cord
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3227
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LOT Information

Cell No.   JCRB1224 Cell Name   UC704
LOT No.   12142007 Lot Specification   distribution
Medium   M061101(within serum MED-SHIROTORI:Cat#61101) Temperature   37 C
Cell Density at Seeding   4.3x10^3 cells/sq.cm Methods for Passages   When the cultures reached subconfluence,cells were harvested after treatment with 0.25% trypsin and 1mM EDTA. and replated with one-fifth of harvested cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   NT
Passage Number   p3 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,13
D13S317:10,12
D7S820:8,11
D16S539:9,11
VWA:14,16
TH01:7
AM:X,Y
TPOX:9,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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