JCRB1301 SC78.H81.C81.A9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1301 Cell Name   SC78.H81.C81.A9
Profile   Mouse hybridoma cell line producing monoclonal antibody against scopolamine. Other Name   
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgG1(k)
Classify   hybridoma Established by   Kikuchi, Y.
Registered by   Kikuchi, Y. Regulation for Distribution   
Comment    Year   2007
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab scopolamine.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J315
Reference
Pubmed id:1367787A monoclonal antibody to scopolamine and its use for competitive enzyme-linked immunosorbent assay.
Kikuchi Y,Irie M,Yoshimatsu K,Ishimaru K,Shimomura K,Satake M,Sueyoshi S,Tanno M,Kamiya S,Sawada J
Phytochemistry. 1991;30(10):3273-6

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LOT Information

Cell No.   JCRB1301 Cell Name   SC78.H81.C81.A9
LOT No.   03032008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Intergen RB52305) Temperature   37 C
Cell Density at Seeding   1.1x10^5 cells/ml Methods for Passages   dilution 1/5( 1/2-1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.8x10^6
Viability at cell freezing (%)   62.9 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1301 Cell Name   SC78.H81.C81.A9
LOT No.   02082021 Lot Specification   distribution
Medium   DMEM(GIBCO：Cat No.;11885-084) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   2.80-3.49x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.38x10^6
Viability at cell freezing (%)   68 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   53.2 Additional information   
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