JCRB1304 MT-45-5-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1304 Cell Name   MT-45-5-3
Profile   Mouse hybridoma cell line producing monoclonal antibody against metallothionein. Other Name   
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgG1(K)
Classify   hybridoma Established by   Kikuchi, Y.
Registered by   kikuchi, Y. Regulation for Distribution   
Comment    Year   2007
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab metallothionein.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J275
Reference
Pubmed id:1500065Identification of metallothionein in cultured cells by immunoblotting and immunofluorescence using a new monoclonal antibody.
Kikuchi Y,Irie M,Ikebuchi H,Sawada J,Terao T
Hybridoma. 1992 Jun;11(3):295-300

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LOT Information

Cell No.   JCRB1304 Cell Name   MT-45-5-3
LOT No.   03282008 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(Intergen Lot.RB52305) Temperature   37 C
Cell Density at Seeding   6.9x10^4 cells/ml Methods for Passages   dilution 1/5(1/2-1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2x10^6
Viability at cell freezing (%)   88.0 Antibiotics Used   NT
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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