JCRB1313 Ra-1 Cell information
Cell type:virus production cells
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JCRB No.
JCRB1313
Cell Name
Ra-1
Profile
Rabbit lymphocyte cell line transformed with HTLV-1.
Other Name
Animal
rabbit
Strain
Genus
Oryctolagus
Species
cuniculus
Sex
Age
Identity
not done
Tissue for Primary Cancer
hemo-lymphocytic
Case history
Rabbit cells were cocultivated with MT-2 cells.
Metastasis
Tissue Metastasized
Genetics
HTLV-1 producing cell line.
Life Span
infinite
Crisis PDL
Morphology
lymphoid
Character
Classify
transformed
Established by
Miyoshi,I.
Registered by
Miyoshi,I.
Regulation for Distribution
Comment
Year
2007
Medium
RPMI 1640 medium with 20% fetal calf serum.
Methods for Passages
dilution
Cell Number on Passage
Split ratio = 1/2 -1/3.
Race
CO2 Conc.
5%
Tissue Sampling
Tissue Type
lymphocyte
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:6301915 Transformation of rabbit lymphocytes with T-cell leukemia virus. Miyoshi I,Yoshimoto S,Taguchi H,Kubonishi I,Fujishita M,Ohtsuki Y,Shiraishi Y,Akagi T Gan. 1983 Feb;74(1):1-4
LOT Information
Cell No.
JCRB1313
Cell Name
Ra-1
LOT No.
07102008
Lot Specification
distribution
Medium
RPMI1640 medium(GIBCO catalog No.11875) with 15% heat inactivated fetal bovine serum(Hyclone GRD0051)
Temperature
37 C
Cell Density at Seeding
4.0x10^5 cells/ml
Methods for Passages
dilution,split ratio 1/2-1/3
Doubling Time
NT
Cell Number in Vial (cells/1ml)
3.8x10^6
Viability at cell freezing (%)
97.7
Antibiotics Used
free
Passage Number
p6*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
No
Exoteric Gene
NT
Medium for Freezing
Cell Banker BLC-1(Nihon Zenyaku Industries)
CO2 Conc.
5 %
Viability immediately after thawing (%)
Additional information