JCRB9072 RD

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9072 Cell Name   RD
Profile   rhabdomyosarcoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   7-year-old
Identity   available Tissue for Primary Cancer   sarcoma
Case history   malignant embryonal rhabdomyosarcoma arose at pelvis Metastasis   
Tissue Metastasized    Genetics   rhabdomyosarcoma
Life Span    Crisis PDL   
Morphology    Character   
Classify    Established by   McAllister,R.M.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL136. Year   1987
Medium   Eagle's minimum essential medium with the twice standard concentration of amino acids and vitamines (in the final concentration, respectively), and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   muscle
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1649
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909264
Reference
Pubmed id:841332 Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:4119036 Radiosensitization of bleomycin on lethal effect of mouse cancer cell in vitro.
Matsuzawa T,Onozawa M,Morita K,Kakehi M
Strahlentherapie. 1972 Nov;144(5):614-7
Pubmed id:4241949 Cultivation in vitro of cells derived from a human rhabdomyosarcoma.
McAllister RM,Melnyk J,Finkelstein JZ,Adams EC Jr,Gardner MB
Cancer. 1969 Sep;24(3):520-6
Pubmed id:4918236 Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus.
Hahon N,Cooke KO
J Virol. 1967 Apr;1(2):317-26

Images
Movies



LOT Information

Cell No.   JCRB9072 Cell Name   RD
LOT No.   01172005 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 2x amino acids, 2x vitamins (in the final conc., respectively), and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   6 - 8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 29 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P42 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9072 Cell Name   RD
LOT No.   122793 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 2X amino acids, 2X vitamins and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1.0x10^6 cells/10cm dish Methods for Passages   Cells are treated with 0.05-0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   P43 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD, G6PD(not HeLa), NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9072 Cell Name   RD
LOT No.   041189 Lot Specification   distribution
Medium   MEM w/ 2x amino acids, 2X vitamins + FCS 10% Temperature   37
Cell Density at Seeding   1/2 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9072 Cell Name   RD
LOT No.   07082013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 2x amino acids, 2X vitamins and 10% fetal calf serum (FBS; GIBCO Cat. # 10099) Temperature   37C
Cell Density at Seeding   5.9 - 7.8 10^4 cells/mL Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.1
Additional information   
Cell No.   JCRB9072 Cell Name   RD
LOT No.   11082016 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 2x amino acids, 2X vitamins and 10% fetal calf serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   82.8 Antibiotics Used   free
Passage Number   P42 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   82.8
Additional information   
CLOSE