JCRB1323 F25

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1323 Cell Name   F25
Profile   Mouse hybridoma cell line producing F25 MAb against CD307 (IRTA2/FcRH5/FCRL5). Other Name   
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgG2a
Classify   hybridoma Established by   Nagata,S.
Registered by   Nagata,S. Regulation for Distribution   
Comment    Year   2007
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (IMDM can be used). Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10). Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab F25.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J279
Reference
Pubmed id:17051241Elevation of soluble CD307 (IRTA2/FcRH5) protein in the blood and expression on malignant cells of patients with multiple myeloma, chronic lymphocytic leukemia, and mantle cell lymphoma.
Ise T,Nagata S,Kreitman RJ,Wilson WH,Wayne AS,Stetler-Stevenson M,Bishop MR,Scheinberg DA,Rassenti L,Kipps TJ,Kyle RA,Jelinek DF,Pastan I
Leukemia. 2007 Jan;21(1):169-74
Pubmed id:16681430Sandwich ELISAs for soluble immunoglobulin superfamily receptor translocation-associated 2 (IRTA2)/FcRH5 (CD307) proteins in human sera.
Ise T,Kreitman RJ,Pastan I,Nagata S
Clin Chem Lab Med. 2006;44(5):594-602
Pubmed id:15671532Immunoglobulin superfamily receptor translocation associated 2 protein on lymphoma cell lines and hairy cell leukemia cells detected by novel monoclonal antibodies.
Ise T,Maeda H,Santora K,Xiang L,Kreitman RJ,Pastan I,Nagata S
Clin Cancer Res. 2005 Jan 1;11(1):87-96
Pubmed id:16157322Characterization of antibodies submitted to the B cell section of the 8th Human Leukocyte Differentiation Antigens Workshop by flow cytometry and immunohistochemistry.
Vidal-Laliena M,Romero X,March S,Requena V,Petriz J,Engel P
Cell Immunol. 2005 Jul-Aug;236(1-2):6-16

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LOT Information

Cell No.   JCRB1323 Cell Name   F25
LOT No.   11282008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.7x10^5 cells/ml Methods for Passages   split 1/5(1/2-1/10)
Doubling Time   Nt Cell Number in Vial (cells/1ml)   8.3x10^6
Viability at cell freezing (%)   77.5 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1323 Cell Name   
LOT No.   12282008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.7 x 10^5 cells/ml Methods for Passages   split 1/5(1/2-1/10)
Doubling Time    Cell Number in Vial (cells/1ml)   8.3 x 10^6
Viability at cell freezing (%)   77.5 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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