JCRB1323 F25
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1323 | Cell Name | F25 |
|---|---|---|---|
| Profile | Mouse hybridoma cell line producing F25 MAb against CD307 (IRTA2/FcRH5/FCRL5). | Other Name | |
| Animal | mouse | Strain | |
| Genus | hybridoma | Species | |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | antibody-producing hybridoma | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Producing IgG2a |
| Classify | hybridoma | Established by | Nagata,S. |
| Registered by | Nagata,S. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (IMDM can be used). | Methods for Passages | dilution |
| Cell Number on Passage | split 1/5 (1/2 - 1/10). | Race | |
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | producing Mab F25. |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:17051241 | Elevation of soluble CD307 (IRTA2/FcRH5) protein in the blood and expression on malignant cells of patients with multiple myeloma, chronic lymphocytic leukemia, and mantle cell lymphoma. Ise T,Nagata S,Kreitman RJ,Wilson WH,Wayne AS,Stetler-Stevenson M,Bishop MR,Scheinberg DA,Rassenti L,Kipps TJ,Kyle RA,Jelinek DF,Pastan I Leukemia. 2007 Jan;21(1):169-74 |
| Pubmed id:16681430 | Sandwich ELISAs for soluble immunoglobulin superfamily receptor translocation-associated 2 (IRTA2)/FcRH5 (CD307) proteins in human sera. Ise T,Kreitman RJ,Pastan I,Nagata S Clin Chem Lab Med. 2006;44(5):594-602 |
| Pubmed id:15671532 | Immunoglobulin superfamily receptor translocation associated 2 protein on lymphoma cell lines and hairy cell leukemia cells detected by novel monoclonal antibodies. Ise T,Maeda H,Santora K,Xiang L,Kreitman RJ,Pastan I,Nagata S Clin Cancer Res. 2005 Jan 1;11(1):87-96 |
| Pubmed id:16157322 | Characterization of antibodies submitted to the B cell section of the 8th Human Leukocyte Differentiation Antigens Workshop by flow cytometry and immunohistochemistry. Vidal-Laliena M,Romero X,March S,Requena V,Petriz J,Engel P Cell Immunol. 2005 Jul-Aug;236(1-2):6-16 |
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LOT Information
| Cell No. | JCRB1323 | Cell Name | F25 |
|---|---|---|---|
| LOT No. | 11282008 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 1.7x10^5 cells/ml | Methods for Passages | split 1/5(1/2-1/10) |
| Doubling Time | Nt | Cell Number in Vial (cells/1ml) | 8.3x10^6 |
| Viability at cell freezing (%) | 77.5 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1323 | Cell Name | |
|---|---|---|---|
| LOT No. | 12282008 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 1.7 x 10^5 cells/ml | Methods for Passages | split 1/5(1/2-1/10) |
| Doubling Time | Cell Number in Vial (cells/1ml) | 8.3 x 10^6 | |
| Viability at cell freezing (%) | 77.5 | Antibiotics Used | free |
| Passage Number | Unknown (5 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |