JCRB1326 NCC-RbC-60

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1326 Cell Name   NCC-RbC-60
Profile   Huaman retinoblastoma cell line. The growth is very slow. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   1.1
Identity   available Tissue for Primary Cancer   
Case history   Primary bilateral retinoblastoma Metastasis   
Tissue Metastasized    Genetics   Bilateral retinoblastoma
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   
Classify   mutant Established by   Inomata, M.
Registered by   Inomata, M. Regulation for Distribution   
Comment    Year   2006
Medium   CMRL1066 medium with 10-15% fetal bovine serum (100 μM Glutamine, 200 μM Sodium pyruvate and 20 μM 2-mercaptoetanol) Methods for Passages   Simple dilution. split ratio=1/5. Subculture every 1 week.
Cell Number on Passage   8 x 10^5 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   primary retinoblastoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4073
Pubmed id:8263010Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol.
Inomata M,Kaneko A,Saijo N,Tokura S
J Cancer Res Clin Oncol. 1994;120(3):149-55
Pubmed id:3115934Chemosensitivity profiles of primary and cultured human retinoblastoma cells in a human tumor clonogenic assay.
Inomata M,Kaneko A
Jpn J Cancer Res. 1987 Aug;78(8):858-68


LOT Information

Cell No.   JCRB1326 Cell Name   NCC-RbC-60
LOT No.   12022008 Lot Specification   distribution
Medium   CMRL1066 medium(GIBCO) with 200uM Glutamine ,200uM Sodium pyruvate,20uM 2-Mercaptoetanol and 15% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.3x10^6 cells/ml Methods for Passages   Suspension culture.Split ratio=1/2.Subculture every 1week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.5x10^6
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information