JCRB1330 Ty-82

Cell information

Important Notice(s)

The adhesion of Ty-82 cells

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1330 Cell Name   Ty-82
Profile   Thymic carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   22
Identity   available Tissue for Primary Cancer   thymus
Case history   Undifferentiated thymic carcinoma Metastasis   
Tissue Metastasized    Genetics   46XX,t(15;19)(q15;p13).
Life Span   infinite Crisis PDL   
Morphology   anaplastic carcinoma cell Character   Thymic carcinoma.
Classify   tumor Established by   Kubonishi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum Methods for Passages   dilution
Cell Number on Passage   Split ratio = 1/2 - 1/3 Race   Japanese
CO2 Conc.   5% Tissue Sampling   pleural effusion
Tissue Type   anaplastic carcinoma cell

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3220
Reference
Pubmed id:1730520Establishment and characterization of a thymic carcinoma cell line (Ty-82) carrying t(15;19)(q15;p13) chromosome abnormality.
Kuzume T,Kubonishi I,Takeuchi S,Takeuchi T,Iwata J,Sonobe H,Ohtsuki Y,Miyoshi I
Int J Cancer. 1992 Jan 21;50(2):259-64

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LOT Information

Cell No.   JCRB1330 Cell Name   
LOT No.   02152010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS, GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   1.3 - 3.9 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^5
Viability at cell freezing (%)   84.3 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1330 Cell Name   Ty-82
LOT No.   02232009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   3.3x10^5 cells/ml Methods for Passages   1/2-1/13
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.6x10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,11
D13S317:11
D7S820:12,13
D16S539:9,11
VWA:17,19
TH01:6,9.3
AM:X
TPOX:9,11
CSF1PO:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1330 Cell Name   Ty-82
LOT No.   09082016 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.9 - 4.3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). Split ratio = 1/2 - 1/3 is recommended.
Doubling Time   approx. 53 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
Cell No.   JCRB1330 Cell Name   Ty-82
LOT No.   03162022 Lot Specification   distribution
Medium   RPMI 1640 medium with heat-inactivated fetal bovine serum (FBS; Biowest Cat. # S18200-500) Temperature   37 C
Cell Density at Seeding   0.5 - 3.2 x 10^5 cells/mL Methods for Passages   Although this cell line is described as suspension culture, sometimes cells will attach to dish. In such case, please use trypsin treatment for harvesting cells.
Doubling Time   55 - 82 hrs. Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   81.7 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   80.0 Additional information   
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