JCRB1222.12 HGH1.31.3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1222.12 | Cell Name | HGH1.31.3 |
|---|---|---|---|
| Profile | Mouse hybridoma cell line producing monoclonal antibody against human growth hormone (hGH). | Other Name | |
| Animal | mouse | Strain | |
| Genus | hybridoma | Species | |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | antibody-producing hybridoma |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Producing IgG gamma1 and k |
| Classify | hybridoma | Established by | Sawada, J. |
| Registered by | Sawada, J. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | dilution |
| Cell Number on Passage | split 1/5 (1/2 - 1/10) | Race | |
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | producing Mab human growth hormone (hGH). |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2427940 | Comparison of the antigenicities of native human growth hormone (hGH) and three forms of recombinant hGHs using monoclonal antibodies. Sawada J,Wada N,Irie M,Tokunaga-Doi T,Ohtsuka E,Ikehara M,Terao T Mol Immunol. 1986 Jun;23(6):625-30 |
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LOT Information
| Cell No. | JCRB1222.12 | Cell Name | HGH1.31.3 |
|---|---|---|---|
| LOT No. | 03062009 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 5.3x10^4 cells/ml | Methods for Passages | split 1/5(1/2-1/10) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.4x10^6 |
| Viability at cell freezing (%) | 90.6 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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