JCRB1332 NALL-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1332 Cell Name   NALL-1
Profile   Human 'null' cell from ALL cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   14
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   Acute lymphocytic leukemia (ALL) Metastasis   
Tissue Metastasized    Genetics   EB virus was not detected. The cell line lacks both T-cell and B-cell markers.
Life Span   infinite Crisis PDL   
Morphology   lymphoblast Character   The cell line is negative for E-rosettes and surface immunoglobulin.
Classify   tumor Established by   Hiraki,S.
Registered by   Miyoshi,I. Regulation for Distribution   Distribution to commercial companies should be informed to Dr. Miyoshi.
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum Methods for Passages   Subculture every 5-7 days. Dilution.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood
Tissue Type   leukemic lymphoblasts

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3039
Reference
Pubmed id:200333Human leukemic "null" cell line (NALL-1).
Hiraki S,Miyoshi I,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Masuji H
Cancer. 1977 Nov;40(5):2131-5
Pubmed id:197412Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias.
Miyoshi I,Hiraki S,Tsubota T,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Kimura I,Masuji H
Nature. 1977 Jun 30;267(5614):843-4

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LOT Information

Cell No.   JCRB1332 Cell Name   
LOT No.   04152011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   5.4 - 6.0 x 10^5 cells/mL Methods for Passages   Simple dilution, passage every 3-4 days.
Doubling Time   approx. 44 hrs. Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPM I1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1332 Cell Name   NALL-1
LOT No.   03272009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   4.3x10^5 cells/ml Methods for Passages   split 1/2-1/13
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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