JCRB9081 L6

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9081 Cell Name   L6
Profile   skeletal muscle myoblast Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   muscular system
Case history    Metastasis   
Tissue Metastasized    Genetics   skeletal muscle myoblast
Life Span    Crisis PDL   
Morphology    Character   skeletal muscle myoblast
Classify    Established by   Yaffe
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1458. Year   1986
Medium   Dulbecco's modified Eagle's medium with 10 % fetal calf serum. Methods for Passages   Cells treated with 0.02% EDTA and 0.25% trypsin & subculture every 2-3 weeks.
Cell Number on Passage   1/4 - 1/16 Race   
CO2 Conc.   5 % Tissue Sampling   muscle
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0385
Reference
Pubmed id:2533272Spectinomycin operon of Micrococcus luteus: evolutionary implications of organization and novel codon usage.
Ohama T,Muto A,Osawa S
J Mol Evol. 1989 Nov;29(5):381-95
Pubmed id:371863[Peptide distribution in the brain].
Yanaihara N,Yanaihara C,Sato H,Shibazaki T,Asami O
Horumon To Rinsho. 1979 Jan;27(1):17-27
Pubmed id:5245982Retention of differentiation potentialities during prolonged cultivation of myogenic cells.
Yaffe D
Proc Natl Acad Sci U S A. 1968 Oct;61(2):477-83

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LOT Information

Cell No.   JCRB9081 Cell Name   
LOT No.   05172012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBD - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9081 Cell Name   
LOT No.   09032009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Pssage twice a week.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   93.9 Antibiotics Used   free
Passage Number   P9 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9081 Cell Name   
LOT No.   10102000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   2-5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P9 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9081 Cell Name   L6
LOT No.   060796 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9081 Cell Name   L6
LOT No.   051492 Lot Specification   distribution
Medium   Dulbecco's EMEM(lot#.032592) + 10% FBS (Cell Culture Lab. lot#.00939-01) Temperature   37 C
Cell Density at Seeding   1.03x10^6cells/10D. Methods for Passages   0.25% trypsin in 0.02% EDTA, change medium or subculture every 3-4 days
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   P8=CORRECT. PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NY
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9081 Cell Name   L6
LOT No.   092791 Lot Specification   distribution
Medium   DMEM + FCS 10% Temperature   37 C
Cell Density at Seeding   1/4 - 1/16 Methods for Passages   0.02% EDTA and 0.25% trypsin. Every 2-3 week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9081 Cell Name   L6
LOT No.   05152017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   approx. 8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   84.5 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   84.5 Additional information   
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