JCRB1334 RC-K8
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1334 | Cell Name | RC-K8 |
|---|---|---|---|
| Profile | A human lymphoma cell line with t(11;14) chromosome abnormality. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 55 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | malignant lymphoma (histiocytic lymphoma) | Metastasis | |
| Tissue Metastasized | Genetics | 46XY, with t(11;14)(q23;q32). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoid | Character | Dt=48-60hr. The cell line produces plasminogen activator. |
| Classify | tumor | Established by | Kubonishi,I. |
| Registered by | Miyoshi,I. | Regulation for Distribution | Distribution to commercial companies should be informed to Dr. Miyoshi. |
| Comment | Year | 2007 | |
| Medium | RPMI 1640 medium with 20% fetal calf serum. | Methods for Passages | dilution |
| Cell Number on Passage | 1/2 - 1/3 | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | chylous peritoneal effusion |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:3742463 | Characterization of a new human lymphoma cell line (RC-K8) with t(11;14) chromosome abnormality. Kubonishi I,Niiya K,Yamashita M,Yano S,Abe T,Ohtsuki Y,Miyoshi I Cancer. 1986 Oct 1;58(7):1453-60 |
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LOT Information
| Cell No. | JCRB1334 | Cell Name | RC-K8 |
|---|---|---|---|
| LOT No. | 04202009 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | NT | Methods for Passages | dilution. Split ratio=1/2-1/3 |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.13x10^6 |
| Viability at cell freezing (%) | 98.7 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,11 D13S317:9 D7S820:10.3,12 D16S539:10 VWA:18,19 TH01:7 AM:X,Y TPOX:8 CSF1PO:11,13 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1334 | Cell Name | RC-K8 |
|---|---|---|---|
| LOT No. | 07052016 | Lot Specification | distribution |
| Medium | RPMI-1640 medium with 20% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 1702012). | Temperature | 37 C |
| Cell Density at Seeding | 2.5 - 9.7 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). |
| Doubling Time | approx. 82 hrs. | Cell Number in Vial (cells/1ml) | 2.0 x 10^6 |
| Viability at cell freezing (%) | 87.0 | Antibiotics Used | free |
| Passage Number | Unknown (7 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 87.0 | Additional information |