JCRB9083 LoVo

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9083 Cell Name   LoVo
Profile   adenocarcinoma, carcinoembryonic antigen-producing Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   colon
Case history    Metastasis   
Tissue Metastasized    Genetics   colon, adenocarcinoma
Life Span    Crisis PDL   
Morphology    Character   colon, adenocarcinoma
Classify    Established by   Drewinko,B. et al.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL229. Year   1986
Medium   Ham's F12 medium with 20% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/2 - 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0399
Reference
Pubmed id:445465 Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives.
Drewinko B,Barlogie B,Freireich EJ
Cancer Res. 1979 Jul;39(7 Pt 1):2630-6
Pubmed id:276641 Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line.
Drewinko B,Yang LY,Barlogie B,Romsdahl M,Meistrich M,Malahy MA,Giovanella B
J Natl Cancer Inst. 1978 Jul;61(1):75-83
Pubmed id:1260746 Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line.
Drewinko B,Romsdahl MM,Yang LY,Ahearn MJ,Trujillo JM
Cancer Res. 1976 Feb;36(2 Pt 1):467-75
Pubmed id:964319 Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells.
Drewinko B,Yand LY
Exp Cell Res. 1976 Sep;101(2):414-6

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LOT Information

Cell No.   JCRB9083 Cell Name   
LOT No.   04282010 Lot Specification   distribution
Medium   Ham's F12 medium with 20% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.4 - 2.2 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days (at log phase) Cell Number in Vial (cells/1ml)   2.6 x 10-6
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9083 Cell Name   
LOT No.   06262002 Lot Specification   distribution
Medium   Ham's F12 medium with 20% fetal bovine serum Temperature   37 C
Cell Density at Seeding    Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4.9 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P35 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9083 Cell Name   LoVo
LOT No.   102595 Lot Specification   distribution
Medium   Ham's F12 medium with 20% fetal bovine serum (JR Scientific DO10400) Temperature   37 C
Cell Density at Seeding   2.5 x 10^5 cells/ml Methods for Passages   Cells are harvested after treated with 0.25% trypsin-0.02% EDTA for 10-15 min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.7x10^6
Viability at cell freezing (%)   88.5 Antibiotics Used   free
Passage Number   P32 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB9083 Cell Name   LoVo
LOT No.   071592 Lot Specification   distribution
Medium   Ham's F12 + FCS 10% (lot.JRN Biosciences 1B1062) Temperature   37
Cell Density at Seeding   2x10^6 Methods for Passages   Cells are washed once with PBS and treated with 0.02 % EDTA and 0.25 % trypsin at 37 C for 15 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.2x10^6
Viability at cell freezing (%)   91.6 Antibiotics Used   free
Passage Number   P31 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9083 Cell Name   LoVo
LOT No.   031789 Lot Specification   distribution
Medium   Ham's F12 + FCS 20% Temperature   37
Cell Density at Seeding   1/2 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P32 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9083 Cell Name   
LOT No.   090397 Lot Specification   distribution
Medium   Ham's F12 medium with 20% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9083 Cell Name   LoVo
LOT No.   06172019 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.8 - 3.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 33 hrs. Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   80.0 Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   76.0
Additional information   
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