JCRB9084 Sp2/O-Ag14

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9084 Cell Name   Sp2/O-Ag14
Profile   myeloma, non-secreting, 8-azaguanine resistant Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   hybridoma, non-secreting
Life Span    Crisis PDL   
Morphology    Character   hybridoma, non-secreting
Classify    Established by   Shulman,M. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL1581. Year   1985
Medium   Dulbecco's modified Eagle's medium with 4.5g/L glucose and 10% fetal calf serum. Methods for Passages   Dilution. A maximum of 10^6/ml is obtainable.
Cell Number on Passage   10^5/ml Race   
CO2 Conc.   5 % Tissue Sampling   myeloma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2199
Reference
Pubmed id:714156 A better cell line for making hybridomas secreting specific antibodies.
Shulman M,Wilde CD,Köhler G
Nature. 1978 Nov 16;276(5685):269-70

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LOT Information

Cell No.   JCRB9084 Cell Name   Sp2/O-Ag14
LOT No.   031194 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum (Hyclone:11151031) Temperature   37 C
Cell Density at Seeding   2.5x10^5 cells/ml Methods for Passages   Dilution. A part of cells are observed to be adhered on the culture dishes loosely.
Doubling Time   dt=22.5 hrs Cell Number in Vial (cells/1ml)   4.1x10^6
Viability at cell freezing (%)   72.5 Antibiotics Used   free
Passage Number   *P6 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP (G6PD not detected), by Okado
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9084 Cell Name   Sp2/O-Ag14
LOT No.   070291 Lot Specification   distribution
Medium   D.EMEM + 10 % FBS, High glucose concentration=4.5 g/L. Temperature   37 C
Cell Density at Seeding   1.2x10^6 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^7
Viability at cell freezing (%)   69.5 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9084 Cell Name   Sp2/O-Ag14
LOT No.   030888 Lot Specification   distribution
Medium   DMEM w/ 4.5g/L glucose + FCS 10% Temperature   37
Cell Density at Seeding   10^5/ml Methods for Passages   Dilution. A maximum of 10^6/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9084 Cell Name   
LOT No.   030598 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   5-10 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time   ca. 22 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9084 Cell Name   Sp2/O-Ag14
LOT No.   07022018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2 - 2.0 x 10^5 cells/mL Methods for Passages   Dilution. A part of cells are observed to be adhered on the culture dishes loosely, and are detached by pipetting.
Doubling Time   approx. 15 hrs. Cell Number in Vial (cells/1ml)   5.7 x 10^6
Viability at cell freezing (%)   66.3 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   66.3
Additional information   
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