JCRB9086 HeLa 229

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9086 Cell Name   HeLa 229
Profile   Subline of the HeLa cell. Other Name   HeLa 229
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   31
Identity   available Tissue for Primary Cancer   uterine cervix
Case history    Metastasis   
Tissue Metastasized    Genetics   epitheloid carcinoma, cervix.
Life Span    Crisis PDL   
Morphology    Character   epitheloid carcinoma, cervix.
Classify   tumor Established by   Jacobs, J.P.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 2.1. Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum. Methods for Passages   Treat with 0.25 % trypsin for 2-4 min at room temperature. Subculture ratio is between 1/2 to 1/6.
Cell Number on Passage   1/2 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 +
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1276
Reference
Pubmed id:11438719Cell culture forensics.
O'Brien SJ
Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7656-8
Pubmed id:9892199Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping.
Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T
Cancer Res. 1999 Jan 1;59(1):141-50
Pubmed id:9215212Record of the first physician to see Henrietta Lacks at the Johns Hopkins Hospital: history of the beginning of the HeLa cell line.
Jones HW Jr
Am J Obstet Gynecol. 1997 Jun;176(6):S227-8
Pubmed id:2754009Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.
Thewessen EA,Freundt I,van Rijsoort-Vos JH,Stolz E,Michel MF,Wagenvoort JH
J Clin Microbiol. 1989 Jun;27(6):1399-400
Pubmed id:3145877Enhancement of yield of Chlamydia trachomatis Hela 229 cell culture.
Wagenvoort JT,van Rijsoort-Vos T,Overkleeft-van de Ree A,Stolz E
Eur J Clin Microbiol Infect Dis. 1988 Dec;7(6):822
Pubmed id:3691964History of the acceptance of human diploid cell strains as substrates for human virus vaccine manufacture.
Hayflick L,Plotkin S,Stevenson RE
Dev Biol Stand. 1987;68():9-17
Pubmed id:14185313ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS.
LEDLEY RS
Science. 1964 Oct 9;146(3641):241-3
Pubmed id:13237281Preservation at subzero temperatures of mouse fibroblasts (strain L) and human epithelial cells (strain HeLa).
SCHERER WF,HOOGASIAN AF
Proc Soc Exp Biol Med. 1954 Nov;87(2):480-7

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LOT Information

Cell No.   JCRB9086 Cell Name   HeLa 229
LOT No.   03152010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non essential amino acids (0.1 mM each) and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   3 - 8 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   92.5 Antibiotics Used   free
Passage Number   P96 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9086 Cell Name   HeLa 229
LOT No.   032792 Lot Specification   distribution
Medium   MEME + non essential A.A + Human serum 20%(lot;Flow Laboratories.29301035.) Temperature   37 C
Cell Density at Seeding   77.2x10^4 Methods for Passages   0.25% trypsin + 0.02% EDTA
Doubling Time   11hr Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   free
Passage Number   P96 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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