JCRB9089 CCRF S-180 II
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9089 | Cell Name | CCRF S-180 II |
|---|---|---|---|
| Profile | S-180 sarcoma derivative | Other Name | sarcoma 180 |
| Animal | mouse | Strain | CFW |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | sarcoma | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | sarcoma 180 derived | |
| Life Span | Crisis PDL | ||
| Morphology | Character | sarcoma 180 | |
| Classify | tumor | Established by | Foley et al. |
| Registered by | Regulation for Distribution | Following rules from ATCC | |
| Comment | Prepared from CCL 8. | Year | 1985 |
| Medium | Eagle's minimal essential medium with non-essential amino acids and 5% calf serum | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin. |
| Cell Number on Passage | 1/2 - 1/6 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | sarcoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:14185313 | ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS. LEDLEY RS Science. 1964 Oct 9;146(3641):241-3 |
| Pubmed id:13823721 | Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines. FOLEY GE,DROLET BP,McCARTHY RE,GOULET KA,DOKOS JM,FILLER DA Cancer Res. 1960 Jul;20():930-9 |
| Pubmed id:13350343 | Sustained propagation of sarcoma of 180 in tissue culture. FOLEY GE,DROLET BP Proc Soc Exp Biol Med. 1956 Jun;92(2):347-52 |
LOT Information
| Cell No. | JCRB9089 | Cell Name | |
|---|---|---|---|
| LOT No. | 07312007 | Lot Specification | distribution |
| Medium | Eagle's minimam essential medium with non-essential amino acids and 5% calf serum | Temperature | 37 C |
| Cell Density at Seeding | 4 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 19 hrs | Cell Number in Vial (cells/1ml) | 3.5 x 10^6 |
| Viability at cell freezing (%) | 96.0 | Antibiotics Used | free |
| Passage Number | P97 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMEO, 20% CS - culture medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9089 | Cell Name | |
|---|---|---|---|
| LOT No. | 041399 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with non-essenti-al amino acids and 5% calf serum | Temperature | 37 C |
| Cell Density at Seeding | 7-8 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | D.T.=ca 16 hrs | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 |
| Viability at cell freezing (%) | 95.0 | Antibiotics Used | free |
| Passage Number | P97 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |