JCRB0117 B104
Cell information
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB0117 | Cell Name | B104 |
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Profile | Human B cell lymphoma cell line with EBNA(-). IgD and IgM were expressed on the surface of cells. | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | Age | child | |
Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
Case history | malignant lymphoma/leukemic st | Metastasis | |
Tissue Metastasized | Genetics | IgM and IgD are expressed on the surface of cells. | |
Life Span | infinite | Crisis PDL | |
Morphology | lymphocyte-like | Character | The cells were killed by anti-IgM but not IgD. |
Classify | tumor | Established by | Mayumi, M. |
Registered by | Mayumi, M. | Regulation for Distribution | free |
Comment | Year | 2009 | |
Medium | RPMI1640 with 10% fetal calf serum supplements, 5 x 10^-5 M 2-mercaptoethanol and 2mM glutamin. (2-ME was not added in current medium for propagation. L-glutamine is included in regular RPMI 1640 medium.) | Methods for Passages | dilution |
Cell Number on Passage | Race | ||
CO2 Conc. | 5% | Tissue Sampling | blood |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
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Pubmed id:1729359 | Anti-IgM antibody-induced cell death in a human B lymphoma cell line, B104, represents a novel programmed cell death. Ishigami T,Kim KM,Horiguchi Y,Higaki Y,Hata D,Heike T,Katamura K,Mayumi M,Mikawa H J Immunol. 1992 Jan 15;148(2):360-8 |
Pubmed id:1988498 | Growth regulation of a human mature B cell line, B104, by anti-IgM and anti-IgD antibodies. Kim KM,Yoshimura T,Watanabe H,Ishigami T,Nambu M,Hata D,Higaki Y,Sasaki M,Tsutsui T,Mayumi M J Immunol. 1991 Feb 1;146(3):819-25 |
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LOT Information
Cell No. | JCRB0117 | Cell Name | B104 |
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LOT No. | 07142009 | Lot Specification | distribution |
Medium | RPMI1640 medium(GIBCO) with 10-20% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
Cell Density at Seeding | 6.0x10^5 cells/ml | Methods for Passages | dilution |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.4x10^6 |
Viability at cell freezing (%) | 96.3 | Antibiotics Used | free |
Passage Number | p4* | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NT |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:10,11 D7S820:12 D16S539:9,11 VWA:16 TH01:7,8 AM:X,Y TPOX:8,11 CSF1PO:12 |
Adhesion | No | Exoteric Gene | NT |
Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
Viability immediately after thawing (%) | Additional information |
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