JCRB0117 B104

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0117 Cell Name   B104
Profile   Human B cell lymphoma cell line with EBNA(-). IgD and IgM were expressed on the surface of cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   child
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   malignant lymphoma/leukemic st Metastasis   
Tissue Metastasized    Genetics   IgM and IgD are expressed on the surface of cells.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   The cells were killed by anti-IgM but not IgD.
Classify   tumor Established by   Mayumi, M.
Registered by   Mayumi, M. Regulation for Distribution   free
Comment    Year   2009
Medium   RPMI1640 with 10% fetal calf serum supplements, 5 x 10^-5 M 2-mercaptoethanol and 2mM glutamin. (2-ME was not added in current medium for propagation. L-glutamine is included in regular RPMI 1640 medium.) Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_M498
Reference
Pubmed id:1729359Anti-IgM antibody-induced cell death in a human B lymphoma cell line, B104, represents a novel programmed cell death.
Ishigami T,Kim KM,Horiguchi Y,Higaki Y,Hata D,Heike T,Katamura K,Mayumi M,Mikawa H
J Immunol. 1992 Jan 15;148(2):360-8
Pubmed id:1988498Growth regulation of a human mature B cell line, B104, by anti-IgM and anti-IgD antibodies.
Kim KM,Yoshimura T,Watanabe H,Ishigami T,Nambu M,Hata D,Higaki Y,Sasaki M,Tsutsui T,Mayumi M
J Immunol. 1991 Feb 1;146(3):819-25

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LOT Information

Cell No.   JCRB0117 Cell Name   B104
LOT No.   07142009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10-20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   6.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.4x10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:10,11
D7S820:12
D16S539:9,11
VWA:16
TH01:7,8
AM:X,Y
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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