JCRB0117 B104
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0117 | Cell Name | B104 |
|---|---|---|---|
| Profile | Human B cell lymphoma cell line with EBNA(-). IgD and IgM were expressed on the surface of cells. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | child | |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | malignant lymphoma/leukemic st | Metastasis | |
| Tissue Metastasized | Genetics | IgM and IgD are expressed on the surface of cells. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | The cells were killed by anti-IgM but not IgD. |
| Classify | tumor | Established by | Mayumi, M. |
| Registered by | Mayumi, M. | Regulation for Distribution | free |
| Comment | Year | 2009 | |
| Medium | RPMI1640 with 10% fetal calf serum supplements, 5 x 10^-5 M 2-mercaptoethanol and 2mM glutamin. (2-ME was not added in current medium for propagation. L-glutamine is included in regular RPMI 1640 medium.) | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1729359 | Anti-IgM antibody-induced cell death in a human B lymphoma cell line, B104, represents a novel programmed cell death. Ishigami T,Kim KM,Horiguchi Y,Higaki Y,Hata D,Heike T,Katamura K,Mayumi M,Mikawa H J Immunol. 1992 Jan 15;148(2):360-8 |
| Pubmed id:1988498 | Growth regulation of a human mature B cell line, B104, by anti-IgM and anti-IgD antibodies. Kim KM,Yoshimura T,Watanabe H,Ishigami T,Nambu M,Hata D,Higaki Y,Sasaki M,Tsutsui T,Mayumi M J Immunol. 1991 Feb 1;146(3):819-25 |
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LOT Information
| Cell No. | JCRB0117 | Cell Name | B104 |
|---|---|---|---|
| LOT No. | 07142009 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10-20% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 6.0x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.4x10^6 |
| Viability at cell freezing (%) | 96.3 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:10,11 D7S820:12 D16S539:9,11 VWA:16 TH01:7,8 AM:X,Y TPOX:8,11 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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