JCRB9090 Ehrlich ascites

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9090 Cell Name   Ehrlich ascites
Profile   Ehrlich-Lettre ascites carcinoma Other Name   strain E
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   ascites
Case history    Metastasis   
Tissue Metastasized    Genetics   Ehrlich-Lettre ascites, carcinoma
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   many floating cells observed
Classify    Established by   Boone, C.W. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 77. Year   1985
Medium   NCTC 135 medium with 10% fetal calf serum Methods for Passages   Mopping or scraping was recommended in the original paper but trypsin treatment is also OK (0.25% trypsin & 0.02% EDTA).
Cell Number on Passage   Dilute cells to 1/3 Race   
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3873
Reference
Pubmed id:4286457 Artificial heterokaryons of animal cells from different species.
Harris H,Watkins JF,Ford CE,Schoefl GI
J Cell Sci. 1966 Mar;1(1):1-30
Pubmed id:14497414 Tissue culture of Ehrlich ascites tumor cells.
SATO J,MATSUDA S,YABE T,USUI K,NODA M
Bull Cancer Inst Okayama Univ Med Sch. 1961 Aug 30;1():42-69

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LOT Information

Cell No.   JCRB9090 Cell Name   Ehrlich ascites
LOT No.   062696 Lot Specification   distribution
Medium   NCTC 135 medium with 10% fetal bovine serum serum(ICN40224115). Temperature   37 C
Cell Density at Seeding    Methods for Passages   Mopping or scraping were recommended in the original paper, but trypsin & EDTA treatment is OK(0.25%trypsin(GIBCO) and 0.02% EDTA)
Doubling Time    Cell Number in Vial (cells/1ml)   1.5x10^6 cells/sq.cm
Viability at cell freezing (%)   96 Antibiotics Used   
Passage Number   p6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB9090 Cell Name   Ehrlich ascites
LOT No.   021291 Lot Specification   distribution
Medium   NCTC 135 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   1/3 Methods for Passages   Mopping or scraping were recommended in the original paper, but trypsin & EDTA treatment is OK(0.25% trypsin,0.02%EDTA).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P5 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB9090 Cell Name   Ehrlich ascites
LOT No.   03052019 Lot Specification   distribution
Medium   NCTC 135 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.3 - 3.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - NCTC 135 medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97.7
Additional information   
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