JCRB1342.09 MOR158.1.3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1342.09 | Cell Name | MOR158.1.3 |
|---|---|---|---|
| Profile | Mouse hybridoma cell line producing monoclonal antibody against morphine. | Other Name | |
| Animal | mouse x mouse | Strain | |
| Genus | hybridoma | Species | |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Producing IgG1 k. |
| Classify | hybridoma | Established by | Sawada, J. |
| Registered by | Sawada, J. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | dilution |
| Cell Number on Passage | split 1/5 (1/2 - 1/10) | Race | |
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | producing Mab morphine. |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:8417377 | Molecular and biochemical analyses of combining sites of monoclonal anti-morphine antibodies. Sawada J,Yamazaki T,Terao T Mol Immunol. 1993 Jan;30(1):77-86 |
| Pubmed id:3211162 | Production and characterization of high-affinity monoclonal antibodies against morphine. Sawada J,Janejai N,Nagamatsu K,Terao T Mol Immunol. 1988 Sep;25(9):937-43 |
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LOT Information
| Cell No. | JCRB1342.09 | Cell Name | MOR158.1.3 |
|---|---|---|---|
| LOT No. | 10162009 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 0.3-1.3x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.34x10^6 |
| Viability at cell freezing (%) | 93.8 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1342.09 | Cell Name | MOR158.1.3 |
|---|---|---|---|
| LOT No. | 01222019 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 1.7 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). The size of cells is small. At the time of centrifugation, 1200 rpm will be adequate. |
| Doubling Time | approx. 25 hrs. | Cell Number in Vial (cells/1ml) | 5.0 x 10^5 |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 64.1 | Additional information |