JCRB1342.10 MOR33.2.9

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1342.10 Cell Name   MOR33.2.9
Profile   Mouse hybridoma cell line producing monoclonal antibody against morphine. Other Name   
Animal   mouse x mouse Strain   
Genus   hyubridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgG1 k.
Classify   hybridoma Established by   Sawada, J.
Registered by   Sawada, J. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab morphine.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J474
Reference
Pubmed id:8417377Molecular and biochemical analyses of combining sites of monoclonal anti-morphine antibodies.
Sawada J,Yamazaki T,Terao T
Mol Immunol. 1993 Jan;30(1):77-86
Pubmed id:3211162Production and characterization of high-affinity monoclonal antibodies against morphine.
Sawada J,Janejai N,Nagamatsu K,Terao T
Mol Immunol. 1988 Sep;25(9):937-43

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LOT Information

Cell No.   JCRB1342.10 Cell Name   MOR33.2.9
LOT No.   11122009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.0-4.0x10^4 cells/ml Methods for Passages   split 1/5(1/2-1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.1x10^6
Viability at cell freezing (%)   88.6 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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