JCRB9092 7D4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9092 Cell Name   7D4
Profile   anti-murine IL-2 receptors (rat antibody) Other Name   
Animal   mouse x rat Strain   
Genus    Species   
Sex    Age   
Identity    Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   
Tissue Metastasized    Genetics   hybridoma, anti-murine IL-2 receptors
Life Span    Crisis PDL   
Morphology    Character   hybridoma, anti-murine IL-2 receptors
Classify    Established by   
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1698. DMEM w/ 4.5g/L glucose, 0.1 mM non essential amino acids, 1 mM sodium pyruvate, 300 micro g/ml L-glutamine. Year   1986
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Dilution. Maintain cultures at cell concentration between 10^5 and 10^6 per ml.
Cell Number on Passage   1-2 x 10^5/ml Race   
CO2 Conc.   5 % Tissue Sampling   hybridoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4497
Reference
Pubmed id:1702762Induction of interleukin-1 and -6 in human gingival fibroblast cultures stimulated with Bacteroides lipopolysaccharides.
Takada H,Mihara J,Morisaki I,Hamada S
Infect Immun. 1991 Jan;59(1):295-301




LOT Information

Cell No.   JCRB9092 Cell Name   7D4
LOT No.   021089 Lot Specification   distribution
Medium   DMEM ( see comments ) + FCS 10% Temperature   37
Cell Density at Seeding   1-2 x 10^5/ml Methods for Passages   Dilution. Maintain cultures at cell concentration between 10^5 and 10^6 per ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P4 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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