JCRB1358.03 SRBC-P20-13-13

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1358.03 Cell Name   SRBC-P20-13-13
Profile   Mouse hybridoma cell line producing monoclonal antibody against sheep erythrocytes (SRBCs). Other Name   
Animal   mouse x mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgM(k). Recloned from P20-13.
Classify   hybridoma Established by   Sawada, J.
Registered by   Sawada, J. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab sheep erythrocytes (SRBCs).

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J494
Pubmed id:3250645Production of monoclonal antibodies to sheep erythrocyte antigens.
Sawada J,Tedao T
Eisei Shikenjo Hokoku. 1988;(106):115-7


LOT Information

Cell No.   JCRB1358.03 Cell Name   SRBC-P20-13-13
LOT No.   01262010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.33-2.79x10^5 cells/ml Methods for Passages   split 1/5(1/2-1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.64x10^6
Viability at cell freezing (%)   77.4 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information