JCRB1362 HCT-2

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1362 Cell Name   HCT-2
Profile   Hamster spleen lymphocytes transformed by human T-cell leukemia virus type I. Other Name   
Animal   hamster, Syrian Strain   Syrian
Genus   Mesocricetus Species   auratus
Sex    Age   
Identity   not done Tissue for Primary Cancer   hemo-lymphocytic
Case history   Lethally irradiated MT-2(HTLV positive) cells were cocultivated with Syrian hamster cells in vitro. Metastasis   
Tissue Metastasized    Genetics   HTLV-1 positive but HTLV-1 is not produced.
Life Span   infinite Crisis PDL   
Morphology    Character   The cells are forming loosely aggregated cell clumps. Hamster T-cell antigen positive. 44, XX(hamster).
Classify   transformed Established by   Akagi,T.
Registered by   Miyoshi.I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage   1/2 - 1/3. Race   
CO2 Conc.   5% Tissue Sampling   spleen
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2920
Pubmed id:3009333Transformation of hamster spleen lymphocytes by human T-cell leukemia virus type I.
Akagi T,Takata H,Ohtsuki Y,Takahashi K,Oka T,Yano S,Miyoshi I
Int J Cancer. 1986 May 15;37(5):775-9


LOT Information

Cell No.   JCRB1362 Cell Name   HCT-2
LOT No.   03012010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.6-2.7x10^5 cells/ml Methods for Passages   1/2-1/3 dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.6x10^6
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information