JCRB1366 GP-1

Cell information

Cell type：virus production cells (View Pricing Information)

JCRB No.   JCRB1366 Cell Name   GP-1
Profile   Guinea pig leukocyte cell line co-infected with HTLV-I and HTLV-II. Other Name   
Animal   guinea pig Strain   Hartley strain
Genus   Cavia Species   porcellus
Sex   F Age   adult
Identity   not done Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   The cell line was established by coinfection of HTLV-I and HTLV-II.
Life Span   infinite Crisis PDL   
Morphology   macrophage-like Character   pol sequences of both HTLV-I and II were detected.
Classify   transformed Established by   Miyoshi,I.
Registered by   MIyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum, 50U/ml interleukin-2. Methods for Passages   dilution
Cell Number on Passage   Split ratio = 1/2 - 1/3. Race   
CO2 Conc.   5% Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2449
Reference
Pubmed id:10195239Transformation of guinea pig leukocytes by coinfection with human T-cell lymphotropic virus types I and II.
Murakami K,Hatakeyama N,Ikezoe T,Kubota T,Kubonishi I,Ohtsuki Y,Sawada T,Miyoshi I
J Hum Virol. 1998 Jan-Feb;1(2):101-6

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LOT Information

Cell No.   JCRB1366 Cell Name   GP-1
LOT No.   04082010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) and IL-2(50 u/ml)(Roche Interleukin-2,human) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   dilution (split ratio=1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   NT
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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