JCRB1367 Vn1919 cl49

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1367 Cell Name   Vn1919 cl49
Profile   neuroglial and neuronal character coexpressing ependymoma cell line. Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   brain
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   GFAP (glial fibrillary acidic protein) positive, NF-L and NF-H (neurofilament) positive.
Classify   tumor Established by   Uchida, S.
Registered by   Aizawa, T. Regulation for Distribution   
Comment    Year   
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   0.05% trypsin, 0.002% EDTA inPBS(-)
Cell Number on Passage   split ratio = 1/8 Race   
CO2 Conc.   5% Tissue Sampling   brain
Tissue Type   ependymoma induced by intracerebroventri

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8542
Pubmed id:21073635Neural stem cell-like gene expression in a mouse ependymoma cell line transformed by human BK polyomavirus.
Aizawa T,Hasegawa K,Ohkumo T,Haga S,Ikeda K,Yoshikawa K
Cancer Sci. 2011 Jan;102(1):122-9
Pubmed id:221713Polyoncogenicity and insulinoma-inducing ability of BK Virus, a human Papovavirus, in Syrian golden hamsters.
Uchida S,Watanabe S,Aizawa T,Furuno A,Muto T
J Natl Cancer Inst. 1979 Jul;63(1):119-26
Pubmed id:191323Induction of papillary ependymomas and insulinomas in the Syrian golden hamster by BK virus, a human papovavirus.
Uchida S,Watanabe S,Aizawa T,Kato K,Furuno A
Gan. 1976 Dec;67(6):857-65


LOT Information

Cell No.   JCRB1367 Cell Name   Vn1919 cl49
LOT No.   03272010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.0-5.0 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.002% EDTA in PBS(-)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.30x10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information