JCRB9095 HEPM

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9095 Cell Name   HEPM
Profile   embryonic palatal mesenchyme, limited life span Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   oral
Case history    Metastasis   
Tissue Metastasized    Genetics   embryonic palatal mesenchyme
Life Span    Crisis PDL   
Morphology    Character   embryonic palatal mesenchyme
Classify    Established by   
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1486. Population doubling was 25 at passage 7. Year   1986
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum (or CS) Methods for Passages   Treat cells with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/2 - 1/16 Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2486
Reference
Pubmed id:7017936Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor.
Yoneda T,Pratt RM
Science. 1981 Jul 31;213(4507):563-5

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LOT Information

Cell No.   JCRB9095 Cell Name   HEPM
LOT No.   072695 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential AAs and 10% fetal bovine serum (JRS.D010400) Temperature   37 C
Cell Density at Seeding   1.4x10^4 cells/sq.cm Methods for Passages   Treat cells with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P12 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:8,12
D7S820:8,10
D16S539:11,12
VWA:17,18
TH01:6,9.3
AM:X
TPOX:8,11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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