JCRB1369 Si-1

Cell information

Cell type：virus production cells (View Pricing Information)

JCRB No.   JCRB1369 Cell Name   Si-1
Profile   Monkey lymphocyte cell line transformed with HTLV-I. Other Name   
Animal   monkey, Japanese macaque Strain   Japanese macaque
Genus   Macaca Species   fuscata
Sex    Age   
Identity   not done Tissue for Primary Cancer   hemo-lymphocytic
Case history   Monkey lymphocyte cells cocultivated with the ATLV producing cell line (MT-2). Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoid Character   Suspension with clumps. Immature lymphoid cells observed. HTLV-I positive.
Classify   transformed Established by   Miyoshi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum, IL-2 100U/mL. Methods for Passages   dilution
Cell Number on Passage   Split ratio = 1/2 - 1/3. Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3165
Reference
Pubmed id:6122826Transformation of monkey lymphocytes with adult T-cell leukaemia virus.
Miyoshi I,Taguchi H,Fujishita M,Yoshimoto S,Kubonishi I,Ohtsuki Y,Shiraishi Y,Akagi T
Lancet. 1982 May 1;1(8279):1016

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LOT Information

Cell No.   JCRB1369 Cell Name   Si-1
LOT No.   04302010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) and IL-2(100 u/ml)(Roche Interleukin-2,human) Temperature   37 C
Cell Density at Seeding   2.6-5.9x10^5 cells/ml Methods for Passages   dilution (split ratio=1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.9x10^6
Viability at cell freezing (%)   95.8 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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