-Detailed Information [JCRB9096]-

JCRB9096 C6

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB9096	Cell Name	C6
Profile	glial tumor, GFAP-positive	Other Name
Animal	rat	Strain
Genus	Rattus	Species	norvegicus
Sex		Age
Identity		Tissue for Primary Cancer	neural
Case history		Metastasis
Tissue Metastasized		Genetics	glial cell tumor
Life Span		Crisis PDL
Morphology		Character	glial cell tumor
Classify		Established by	Benda,P. et al.
Registered by		Regulation for Distribution
Comment	Prepared from CCL 107.	Year	1986
Medium	Ham's F10 medium 82.5% with 15% horse serum and 2.5% fetal calf serum.	Methods for Passages	Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage	1/2 - 1/3	Race
CO2 Conc.	5 %	Tissue Sampling	brain
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	not tested				Detected RNA Virus	not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0194

Reference
Pubmed id:4873531	Differentiated rat glial cell strain in tissue culture. Benda P,Lightbody J,Sato G,Levine L,Sweet W Science. 1968 Jul 26;161(3839):370-1

Images

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LOT Information

031596
052295
011193
040689
09112000
10102014

Cell No.	JCRB9096	Cell Name	C6
LOT No.	031596	Lot Specification	distribution
Medium	Ham's F10 with 15% horse serum (lot;ICN Flow 2070033) and 2.5% fetal calf serum (lot;JRS CO19407)	Temperature	37 C
Cell Density at Seeding	1-2 x 10^5 cells/ml	Methods for Passages	Wash with PBS(-), and treat with 0.25% trypsin and0.02% EDTA for 5 min at 37 C.
Doubling Time		Cell Number in Vial (cells/1ml)	1.5 x 10^6
Viability at cell freezing (%)	97	Antibiotics Used	free
Passage Number	P44	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing		CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB9096	Cell Name	C6
LOT No.	052295	Lot Specification	distribution
Medium	HamF10 with 15% horse serum (ICN Flow 2070033) and 2.5% fetal bovine serum (ICN Flow 101083)	Temperature	37 C
Cell Density at Seeding	1.0x10^6 cells/dish	Methods for Passages	Wash cells with PBS(-) twice and treated with 0.25% trypsin and 0.02% EDTA for 3 min. at room tmp.
Doubling Time	16.2 hr	Cell Number in Vial (cells/1ml)	1.11x10^6
Viability at cell freezing (%)	98.0	Antibiotics Used	free
Passage Number	P43	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	G6PD,LDH,NP
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9096	Cell Name	C6
LOT No.	011193	Lot Specification	distribution
Medium	Ham's F10 medium (Gibco) with 15% horse serum (ICNFlow 0130293) and 2.5% FBS (Flow, 0070893)	Temperature	37 C
Cell Density at Seeding	1.2x10^5cells/ml	Methods for Passages	Cells are washed once with PBS(-) and treated with0.25% trypsin and 0.02% EDTA for 3min at room tmp.
Doubling Time	12-24 hr.	Cell Number in Vial (cells/1ml)	1.98x10^6
Viability at cell freezing (%)	98%	Antibiotics Used	free
Passage Number	P44	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD,NP
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9096	Cell Name	C6
LOT No.	040689	Lot Specification	distribution
Medium	Ham's F10, 82.5% + horse serum, 15% + FCS, 2.5%	Temperature	37
Cell Density at Seeding	1/2 - 1/3	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	5.8x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P44	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9096	Cell Name
LOT No.	09112000	Lot Specification	distribution
Medium	Ham's F10 medium with 15% horse serum and 2.5% fetal calf serum	Temperature	37 C
Cell Density at Seeding	3-6 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	D.T. = 26-29 hrs	Cell Number in Vial (cells/1ml)	4.0 x 10^6
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	P45	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as rat by NP, G6PD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9096	Cell Name	C6
LOT No.	10102014	Lot Specification	distribution
Medium	Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	1.0 - 1.7 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 19 hrs	Cell Number in Vial (cells/1ml)	1.9 x 10^6
Viability at cell freezing (%)	75.8	Antibiotics Used	free
Passage Number	P43	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as rat by NP, G6PD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO in culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	75.8	Additional information