JCRB9097 I-10

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9097 Cell Name   I-10
Profile   Leydig cell testicular tumor (BALB/cJ mouse) Other Name   
Animal   mouse Strain   BALB/cJ
Genus   Mus Species   musclus
Sex   M Age   
Identity    Tissue for Primary Cancer   testis
Case history   leydig cell testicular tumor Metastasis   
Tissue Metastasized    Genetics   leydig cell testicular tumor
Life Span   infinite Crisis PDL   
Morphology    Character   This cell line is a clone of leydig cell testicular tumor H10119. Produces steroids such as progesterone and its reduced derivative.
Classify    Established by   Yasumura,Y. et al.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL 83. Year   1986
Medium   Ham's F10 medium 82.5% with 15% horse serum and 2.5% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/4 - 1/16 Race   
CO2 Conc.   5 % Tissue Sampling   testis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3527
Reference
Pubmed id:4382264Studies on interstitial cells in tissue culture: steroid biosynthesis in monolayers of mouse testicular interstitial cells.
Shin SI
Endocrinology. 1967 Sep;81(3):440-8

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LOT Information

Cell No.   JCRB9097 Cell Name   
LOT No.   04302004 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   0.5 - 1.0 x 10^5 cells/ml Methods for Passages   Collect the floating cells as well as attached cells harvested after treatment with 0.25% trypsin and 0.02% EDTA, and re-suspend them to fresh medium.
Doubling Time   ca. 40 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P42 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9097 Cell Name   I-10
LOT No.   051989 Lot Specification   distribution
Medium   Ham's F10, 82.5% + horse serum, 15% + FCS, 2.5% Temperature   37
Cell Density at Seeding   1/4 - 1/16 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9097 Cell Name   I-10
LOT No.   07132015 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   approx. 10^5 cells/mL Methods for Passages   Collect the floating cells as well as attached cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA, and re-suspend them to fresh medium.
Doubling Time   approx. 42 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   62 Antibiotics Used   free
Passage Number   P42 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 15% HS, 2.5% FBS - Ham's F10 CO2 Conc.   5%
Viability immediately after thawing (%)   62 Additional information   
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