JCRB9098 AtT-20

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9098 Cell Name   AtT-20
Profile   pituitary tumor, ACTH producing, (LAF1 mouse) Other Name   
Animal   mouse Strain   LAF1
Genus   Mus Species   Musclus
Sex    Age   
Identity    Tissue for Primary Cancer   pituitary
Case history   pituitary tumor Metastasis   
Tissue Metastasized    Genetics   ACTH producing cell line
Life Span   infinite Crisis PDL   
Morphology   small round cells Character   Cells are subcultured as intact aggregates.
Classify    Established by   Sato,G.
Registered by    Regulation for Distribution   Cells were originally derived from ATCC to JCRB directly.
Comment   Prepared from CCL 89. The cell line grows as floating clusters of cells. Year   1986
Medium   Ham's F10 medium 82.5% with 15% horse serum and 2.5% fetal calf serum. Methods for Passages   Dilution.
Cell Number on Passage   1/4 - 1/16 Race   
CO2 Conc.   5 % Tissue Sampling   pituitary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2300
Reference
Pubmed id:13874682Hormone-producing cultures of adrenal and pituitary tumor origin.
BUONASSISI V,SATO G,COHEN AI
Proc Natl Acad Sci U S A. 1962 Jul 15;48():1184-90
Pubmed id:13121002ACTH secreting transplantable pituitary tumors.
FURTH J,GADSEN EL,UPTON AC
Proc Soc Exp Biol Med. 1953 Oct;84(1):253-4

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LOT Information

Cell No.   JCRB9098 Cell Name   
LOT No.   11152004 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   roughly 1 x 10^5 cells/ml Methods for Passages   Dissociate cell aggregates into small flagments by pipetting and dilute to fresh medium (trypsin digestion is also possible).
Doubling Time   ca. 3 days Cell Number in Vial (cells/1ml)   1.3 x 10^7
Viability at cell freezing (%)   89.7 Antibiotics Used   free
Passage Number   P51 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 15% horse serum, 2.5% fetal bovine serum - Ham's F10 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9098 Cell Name   AtT-20
LOT No.   083194 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum(ICN Flow,lot.207033) and 2.5% fetal bovine serum(JRHB.1B1062) Temperature   37 C
Cell Density at Seeding   Hard to determine(*) Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   uncontable
Viability at cell freezing (%)   ND Antibiotics Used   free
Passage Number   P55 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LDH,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   Cell growth is very slow.
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9098 Cell Name   AtT-20
LOT No.   102290 Lot Specification   distribution
Medium   Ham's F10, 82.5% + horse serum, 15% + FCS, 2.5% Temperature   37
Cell Density at Seeding   1/4 - 1/16 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P52 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9098 Cell Name   AtT-20
LOT No.   072588 Lot Specification   distribution
Medium   Ham's F10, 82.5% + horse serum, 15% + FCS, 2.5% Temperature   37
Cell Density at Seeding   1/4 - 1/16 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P52 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9098 Cell Name   AtT-20
LOT No.   08262014 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   91.8 Antibiotics Used   free
Passage Number   P52 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   91.8 Additional information   
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