JCRB1384 STR-428

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1384 Cell Name   STR-428
Profile   Human HHV-8 negative malignant effusion lymphoma (DLBCL). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   53
Identity   available Tissue for Primary Cancer   ascites
Case history   Ascites lymphoma Metastasis   
Tissue Metastasized    Genetics   IgH, c-Myc, BCL2 rearrangements, complicated karyotypes including t(14:18)(q32;q21), der(8)t(3;8)(q27;q24)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Immunophenotypic characterization; CD3-,CD4-,CD5-,CD8-,CD10+,CD19+,CD20+,CD22+,CD30-,CD38+,HLA-DR+, Viral status; HIV(-), HHV-8(-), EBV(-).
Classify   tumor Established by   Tomoyose,T.
Registered by   Masuda,M. Regulation for Distribution   
Comment   Contact to Dr. Taira,T. to get information of this cell line. Year   2007
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   *
Cell Number on Passage   * Race   Japanese
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   large B cell lymphoma (DLBC)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8533
Reference
Pubmed id:17306873Lack of mitochondrial depolarization by oxidative stress is associated with resistance to buthionine sulfoximine in acute lymphoblastic leukemia cells.
Goto H,Yanagimachi M,Kajiwara R,Kuroki F,Yokota S
Leuk Res. 2007 Sep;31(9):1293-301

Images



LOT Information

Cell No.   JCRB1384 Cell Name   STR-428
LOT No.   12092010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.44-2.4x10^5 cells/ml Methods for Passages   subculture by dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1x10^6
Viability at cell freezing (%)   78.4 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11,12
D7S820:11
D16S539:9,12
VWA:14
TH01:6,9
AM:X,Y
TPOX:8
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1384 Cell Name   STR-428
LOT No.   05182020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.5 - 5.2 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). Cells will form aggregates. At passage, please disperse the cells by pipetting.
Doubling Time   approx. 15 hrs. Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   90.6 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   87.3 Additional information   
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