JCRB1385 CAS-9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1385 Cell Name   CAS-9
Profile   Hybridoma cell line producing antibody against mouse CD49d (VLA-4 a chain). Binding inhibition VLA-4/Fibronectin. Other Name   
Animal   mouse x rat Strain   
Genus   Hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing rat IgG 2b and kappa
Classify   hybridoma Established by   Kina T.
Registered by   Kina T. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI-1640 medium (10% FBS, 2mM Glutamine, 50uM 2-ME) Methods for Passages   dilution
Cell Number on Passage   Split 1/20 (1/10 - 1/20) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   Producing Mab mouse CD49d (VLA-4 a chain)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8537
Reference
Pubmed id:8660794Requirement of cell interactions through adhesion molecules in the early phase of T cell development.
Wada K,Kina T,Kawamoto H,Kondo M,Katsura Y
Cell Immunol. 1996 May 25;170(1):11-9

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LOT Information

Cell No.   JCRB1385 Cell Name   CAS-9
LOT No.   12092010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 2mM Glutamine,50uM　2-ME and 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.418-1.61x10^5 cells/ml Methods for Passages   Split 1/20(1/10-1/20)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.54x10^6
Viability at cell freezing (%)   85.9 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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