JCRB1391 KMS-33
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1391 | Cell Name | KMS-33 |
|---|---|---|---|
| Profile | human myeloma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 70 |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | small round-cell morphology, part is multinucleate cell. Feeder cells (mouse fibloblast) treated with Mitomycin-C (#PMEF-H;Millipore) and IL-6 are necessary for proliferation. |
| Classify | tumor | Established by | Otsuki, T. |
| Registered by | Otsuki, T. | Regulation for Distribution | |
| Comment | Year | 2006 | |
| Medium | RPMI1640 medium with 10% featal bovine serum + rHu IL-6 (10-100ng/mL). | Methods for Passages | dilution(on feeder layer). |
| Cell Number on Passage | 1-4 x 10^5 cells/mL | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | bone marrow |
| Tissue Type | myeloma(IgGλ) |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15491288 | Expression of protein gene product 9.5 (PGP9.5)/ubiquitin-C-terminal hydrolase 1 (UCHL-1) in human myeloma cells. Otsuki T,Yata K,Takata-Tomokuni A,Hyodoh F,Miura Y,Sakaguchi H,Hatayama T,Hatada S,Tsujioka T,Sato Y,Murakami H,Sadahira Y,Sugihara T Br J Haematol. 2004 Nov;127(3):292-8 |
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LOT Information
| Cell No. | JCRB1391 | Cell Name | KMS-33 |
|---|---|---|---|
| LOT No. | 01312011 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) and recombinant human IL-6(10-100ng/ml). Requires the cultivation on mitomycin-treated MEFs' feeder layer. | Temperature | 37 C |
| Cell Density at Seeding | 1-4x10^5 cells/ml | Methods for Passages | Dilution (on mitomycin-treated MEFs' feeder layer) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.68x10^6 |
| Viability at cell freezing (%) | 85.9 | Antibiotics Used | free |
| Passage Number | p12* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:14 D7S820:10,12 D16S539:10 VWA:14,18 TH01:9 AM:X TPOX:8,11 CSF1PO:11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1391 | Cell Name | KMS-33 |
|---|---|---|---|
| LOT No. | 06242024 | Lot Specification | distribution |
| Medium | RPMI1640 medium (GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA, Cat#172012, Lot#12J396) and r hu IL-6(10-100 ng/ml) (Pepro Tech,cat No. 200-06) | Temperature | 37 C |
| Cell Density at Seeding | 2-2.5x10^5 cells/ml | Methods for Passages | dilution (on feeder; mitomycin C treated MEF) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.23x10^6 |
| Viability at cell freezing (%) | 92.5 | Antibiotics Used | 76.0 |
| Passage Number | p13* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:14 D7S820:10,12 D16S539:10 VWA:14,18 TH01:9 AM:X TPOX:8,11 CSF1PO:11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 76.0 | Additional information | IL-6 and feeder cells are necessary for proliferation. |