JCRB9105 P3X63Ag8

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB9105 Cell Name   P3X63Ag8
Profile   myeloma, plasmacytoma, IgG1(gamma,kappa) secreting, (BALB/c mouse) Other Name   
Animal   mouse Strain   BALB/C
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   resistant to 10^-4M 8-azaguanine,die in HAT medium
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   IgG1(gamma,kappa) secreting myeloma
Classify   transformed Established by   Kohler,G. et al.
Registered by    Regulation for Distribution   
Comment   Prepared from TIB 9. Year   1987
Medium   Dulbecco's modified Eagle's medium with 4.5g/L glucose and 10% fetal calf serum. Methods for Passages   Dilution. A maximum of 1-2 x 10^6/ml is obtainable.
Cell Number on Passage   1-2 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   MOPC-21 plasmacytoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3411
Pubmed id:113458A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
Kearney JF,Radbruch A,Liesegang B,Rajewsky K
J Immunol. 1979 Oct;123(4):1548-50
Pubmed id:74805Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse.
Lemke H,Hammerling GJ,Hohmann C,Rajewsky K
Nature. 1978 Jan 19;271(5642):249-51
Pubmed id:268647Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.
Koprowski H,Gerhard W,Croce CM
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8
Pubmed id:305123Fusion of T and B cells.
Köhler G,Pearson T,Milstein C
Somatic Cell Genet. 1977 May;3(3):303-12
Pubmed id:1172191Continuous cultures of fused cells secreting antibody of predefined specificity.
Köhler G,Milstein C
Nature. 1975 Aug 7;256(5517):495-7
Pubmed id:5439579Mouse myelomas and lymphomas in culture.
Horibata K,Harris AW
Exp Cell Res. 1970 Apr;60(1):61-77


LOT Information

Cell No.   JCRB9105 Cell Name   P3X63Ag8
LOT No.   120795 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Flow 0070893) Temperature   37 C
Cell Density at Seeding   1.1x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   92.4 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information