JCRB9105 P3X63Ag8
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9105 | Cell Name | P3X63Ag8 |
|---|---|---|---|
| Profile | myeloma, plasmacytoma, IgG1(gamma,kappa) secreting, (BALB/c mouse) | Other Name | |
| Animal | mouse | Strain | BALB/C |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | resistant to 10^-4M 8-azaguanine,die in HAT medium | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | IgG1(gamma,kappa) secreting myeloma |
| Classify | transformed | Established by | Kohler,G. et al. |
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from TIB 9. | Year | 1987 |
| Medium | Dulbecco's modified Eagle's medium with 4.5g/L glucose and 10% fetal calf serum. | Methods for Passages | Dilution. A maximum of 1-2 x 10^6/ml is obtainable. |
| Cell Number on Passage | 1-2 x 10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | MOPC-21 plasmacytoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:113458 | A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. Kearney JF,Radbruch A,Liesegang B,Rajewsky K J Immunol. 1979 Oct;123(4):1548-50 |
| Pubmed id:74805 | Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Lemke H,Hammerling GJ,Hohmann C,Rajewsky K Nature. 1978 Jan 19;271(5642):249-51 |
| Pubmed id:268647 | Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Koprowski H,Gerhard W,Croce CM Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8 |
| Pubmed id:305123 | Fusion of T and B cells. Köhler G,Pearson T,Milstein C Somatic Cell Genet. 1977 May;3(3):303-12 |
| Pubmed id:1172191 | Continuous cultures of fused cells secreting antibody of predefined specificity. Köhler G,Milstein C Nature. 1975 Aug 7;256(5517):495-7 |
| Pubmed id:5439579 | Mouse myelomas and lymphomas in culture. Horibata K,Harris AW Exp Cell Res. 1970 Apr;60(1):61-77 |
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LOT Information
| Cell No. | JCRB9105 | Cell Name | P3X63Ag8 |
|---|---|---|---|
| LOT No. | 120795 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Flow 0070893) | Temperature | 37 C |
| Cell Density at Seeding | 1.1x10^5 cells/ml | Methods for Passages | Simple dilution. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.4x10^6 |
| Viability at cell freezing (%) | 92.4 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
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