JCRB1413 ST 13

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1413 Cell Name   ST 13
Profile   Mouse normal preadipocyte cell line established from ddN mouse. ST 13 can be induced to differentiate into fat-accumulating cells in vitro. Other Name   
Animal   mouse Strain   ddN
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   differentiate to adipocyte
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Sato, M.
Registered by   Sato, M. Regulation for Distribution   
Comment    Year   2010
Medium   HAM:DMEM(1:1) medium with 10 % fetal calf serum Methods for Passages   0.02% EDTA and 0.25% Trypsin.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   mammary gland
Tissue Type   normal

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8581
Reference
Pubmed id:16857754Calpain system regulates the differentiation of adult primitive mesenchymal ST-13 adipocytes.
Yajima Y,Sato M,Sorimachi H,Inomata M,Maki M,Kawashima S
Endocrinology. 2006 Oct;147(10):4811-9
Pubmed id:12746319Mechanism of adult primitive mesenchymal ST-13 preadipocyte differentiation.
Yajima Y,Sato M,Sumida M,Kawashima S
Endocrinology. 2003 Jun;144(6):2559-65
Pubmed id:11824563Dehydrotrametenolic acid induces preadipocyte differentiation and sensitizes animal models of noninsulin-dependent diabetes mellitus to insulin.
Sato M,Tai T,Nunoura Y,Yajima Y,Kawashima S,Tanaka K
Biol Pharm Bull. 2002 Jan;25(1):81-6
Pubmed id:3275678Preadipocyte differentiation in vitro: identification of a highly active adipogenic agent.
Hiragun A,Sato M,Mitsui H
J Cell Physiol. 1988 Jan;134(1):124-30
Pubmed id:2982429Differentiation-associated increase of cAMP-dependent type II protein kinase in a murine preadipose cell line (ST 13).
Sato M,Hiragun A,Mitsui H
Biochim Biophys Acta. 1985 Mar 21;844(3):296-305
Pubmed id:6998485Preadipocytes possess cellular retinoid binding proteins and their differentiation is inhibited by retinoids.
Sato M,Hiragun A,Mitsui H
Biochem Biophys Res Commun. 1980 Aug 29;95(4):1839-45
Pubmed id:6998859Establishment of a clonal cell line that differentiates into adipose cells in vitro.
Hiragun A,Sato M,Mitsui H
In Vitro. 1980 Aug;16(8):685-93

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LOT Information

Cell No.   JCRB1413 Cell Name   ST 13
LOT No.   02162012 Lot Specification   distribution
Medium   DMEM/ F12(1/1) medium(GIBCO 11320-033) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.53-2.1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.06x10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   
Passage Number   p14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1413 Cell Name   ST 13
LOT No.   08082024 Lot Specification   distribution
Medium   DMEM/F12 with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   3.0 - 3.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   24 - 34 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   99.4 Antibiotics Used   free
Passage Number   Unknown (17 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   97.6 Additional information   
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