JCRB1414 A549-Luc

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1414 Cell Name   A549-Luc
Profile   Luciferase stably expressing cell line (A549; Human Non-small cell lung cancer). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   58
Identity   available Tissue for Primary Cancer   
Case history   carcinoma Metastasis   Yes
Tissue Metastasized    Genetics   pMSCV-luc transfected A549 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   puromycin-resistant
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2009
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   2x10^4 cells/sq.cm. Race   Caucasian
CO2 Conc.   5% Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J242
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LOT Information

Cell No.   JCRB1414 Cell Name   A549-Luc
LOT No.   06212011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) and non essential amino acids(GIBCO) Temperature   37 C
Cell Density at Seeding   5.0-6.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3-1/8)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.11x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   NT
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:8,11
D16S539:11,12
VWA:14
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1414 Cell Name   A549-Luc
LOT No.   10232014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(SIGMA12J3961) and non essential amino acids(GIBCO) Temperature   37 C
Cell Density at Seeding   3.3-5.1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.10x10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:8,11
D16S539:11,12
VWA:14
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   NT
Additional information   
Images
Cell No.   JCRB1414 Cell Name   A549-Luc
LOT No.   11212019 Lot Specification   distribution
Medium   Eagle's minimum essential medium (GIBCO Cat#11095) with 10% heat inactivated fetal bovine serum(SIGMA Cat#172012,Lot#12J396) and non essential amino acids(GIBCO,Cat#11140) Temperature   37 C
Cell Density at Seeding   2.11x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.10x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:8,11
D16S539:11,12
VWA:14
TH01:8,9.3
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95
Additional information   
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