JCRB1415 KasumiA-568
Cell information
Important Notice(s)Procedure for addition of GM-CSF
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1415 | Cell Name | KasumiA-568 |
|---|---|---|---|
| Profile | myeloblastic leukemia | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 65 |
| Identity | available | Tissue for Primary Cancer | |
| Case history | acute myeloid leukemia (FABM6) | Metastasis | |
| Tissue Metastasized | Genetics | 45,XY,del(2)(q?),-13,-16,-19,add(19)(q13.1),add(21)(q22),+mar1,+mar2 | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | CD13+, CD33+, c-kit+, GPA+ |
| Classify | tumor | Established by | Asou, H. |
| Registered by | Asou, H | Regulation for Distribution | |
| Comment | Year | 2009 | |
| Medium | RPMI1640 medium with 20% featal bovine serum and GM-CSF (10ng/mL). | Methods for Passages | dilution (split ratio 1:2) |
| Cell Number on Passage | 0.5-2.0 x 10^6 cells | Race | |
| CO2 Conc. | 5% | Tissue Sampling | blood |
| Tissue Type | tumor |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1415 | Cell Name | KasumiA-568 |
|---|---|---|---|
| LOT No. | 07042011 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) and 10ng/ml rhu GM-CSF. | Temperature | 37 C |
| Cell Density at Seeding | 0.25-1.0x10^6 cells/ml | Methods for Passages | Dilution (split ratio=1/2) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.60x10^6 |
| Viability at cell freezing (%) | 91.2 | Antibiotics Used | free |
| Passage Number | p7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,11 D13S317:8,12 D7S820:12 D16S539:9,11 VWA:17 TH01:7 AM:X,Y TPOX:8 CSF1PO:12,13 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1415 | Cell Name | KasumiA-568 |
|---|---|---|---|
| LOT No. | 10192018 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) and GM-CSF (10ng/mL). | Temperature | 37 C |
| Cell Density at Seeding | 2.0-3.0 x 10^5 cells | Methods for Passages | dilution (split ratio 1/2-1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.5x10^6 |
| Viability at cell freezing (%) | 96.1 | Antibiotics Used | free |
| Passage Number | p9* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,11 D13S317:8,12 D7S820:12 D16S539:9,11 VWA:17 TH01:7,9 AM:X,Y TPOX:8 CSF1PO:12,13 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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