JCRB1416 KasumiA-554
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1416 | Cell Name | KasumiA-554 |
|---|---|---|---|
| Profile | myeloid leukemia secondary to ATL | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 87 |
| Identity | available | Tissue for Primary Cancer | |
| Case history | adult T cell leukemia => secondary leukemia | Metastasis | |
| Tissue Metastasized | Genetics | t(11;19)(q23;p13.1), MLL gene rearrangement, no HTLV1 integration | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | CD33+, CD15+, CD4+, CD56+, HLA-DR+ |
| Classify | tumor | Established by | Asou, H. |
| Registered by | Asou, H. | Regulation for Distribution | |
| Comment | Year | 2009 | |
| Medium | RPMI1640 medium with 20% featal bovine serum. | Methods for Passages | dilution (split ratio 1:2) |
| Cell Number on Passage | 0.5-2.0 x 10^6 cells | Race | |
| CO2 Conc. | 5% | Tissue Sampling | blood |
| Tissue Type | tumor |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1416 | Cell Name | KasumiA-554 |
|---|---|---|---|
| LOT No. | 07072011 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.4-2.2x10^6 cells/ml | Methods for Passages | Dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.7x10^6 |
| Viability at cell freezing (%) | 97.2 | Antibiotics Used | free |
| Passage Number | p6* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:12 D7S820:11 D16S539:9,12 VWA:16,18 TH01:6,9 AM:X TPOX:11,12 CSF1PO:10,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1416 | Cell Name | KasumiA-554 |
|---|---|---|---|
| LOT No. | 02082019 | Lot Specification | distribution |
| Medium | RPMI-1640 medium with 20% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 1.0 - 2.0 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). |
| Doubling Time | approx. 45 hrs. | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 |
| Viability at cell freezing (%) | 88.9 | Antibiotics Used | free |
| Passage Number | Unknown (9 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 88.7 | Additional information |