-Detailed Information [JCRB1418]-

JCRB1418 KYSE790

Cell information

Important Notice(s)

当該細胞株は、利用者が私企業に所属する場合、京都大学との間で個別にマテリアルトランスファーアグリーメント (MTA) を取り交わすよう依頼を受けております。従って、企業ユーザーの方は分譲の依頼に先立ち、下記、細胞樹立者の嶋田先生に連絡して京都大学との間でＭＴＡを取り交わし、そのコピーを貼付して分譲依頼を行ってください。その際、実験目的等の情報についてお尋ねする場合があります。

なお、嶋田先生に連絡をする際、ＪＣＲＢ番号、細胞名を明確にお伝えください。

連絡先：

〒606-8501
京都市左京区吉田下阿達町46-29
京都大学薬学研究科
ナノバイオ医薬創成科学
嶋田 裕
Tel: 075-753-9558
Fax: 075-753-9557
yshimada@pharm.kyoto-u.ac.jp

これは京都大学の基準が適応されてされております。
アカデミックユーザーの場合はこの取扱の必要はありません。直接分譲依頼をしてください。


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KYSEシリーズ細胞株の使用について


−　KYSEシリーズ 食道がん由来細胞株の使用について　−

１．KYSEシリーズ細胞株の分譲は日本国内に限定することとなりました。

２．KYSEシリーズ細胞株の分譲に際しては、別途お送りする「細胞株の使用に関する同意書」にて次の事項に同意していただきます。
　−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−
　(1)．当該細胞株を用いた発表論文には、次に指定する細胞株の樹立に関する原著論文
　　　 （当該細胞に関するもの）を引用する（ただし、引用論文数に制約等があり、
　　　 引用することが困難な場合はこの限りではない）。



　　　　Shimada Y, Imamura M, Wagata T, Yamaguchi N and Tobe T.
　　　　Characterization of twenty one newly established esophageal cancer
　　　　cell lines.　Cancer, 69: 277-284,(1992）

　　　　Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K.
　　　　Characterization of p53 gene mutations in esophageal squamous cell 
　　　　carcinoma cell lines: increased frequency and different spectrum 
　　　　of mutations from primary tumors.
　　　　Int J Cancer. 65:372-376 (1996)

　　　　Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K.
　　　　Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) 
　　　　genes in 30 esophageal squamous-cell-carcinoma cell lines.
　　　　Int J Cancer. 70:437-442 (1997)


　(2)．当該細胞株を用いて研究成果が得られた場合（論文や発表学会など）には、別刷り
　　　（あるいは論文のPDF)、学会要旨のコピー等を細胞株樹立者へ送付する。

　(3)．当該細胞株の第三者への配布は固く禁止する。
　−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−

３．KYSEシリーズ細胞株の民間企業への分譲に際しては、「細胞株の使用に関する同意書」とは別に、京都大学とのMTAの手交が
　　必要です。

(2014年12月15日 更新)

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Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

- Unavailability of KYSE series cell lines to oversea users outside of Japan -

The distribution of KYSE series esophageal cancer cell lines is limited to users in Japan. The JCRB/HSRRB cell bank is sorry for the unavailability of these cell lines to oversea researchers.

(Last updated May 14, 2008.)


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Cell type：general cells (View Pricing Information)

JCRB No.	JCRB1418	Cell Name	KYSE790
Profile	Human well differentiated squamous cell carcinoma cell line established from esophageal cancer.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	68
Identity	available	Tissue for Primary Cancer	esophagus
Case history	well differentiated squamous cell carcinoma, stage 4	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	T2M1N1 stage 4
Classify	tumor	Established by	Shimada, Y.
Registered by	Shimada, Y.	Regulation for Distribution
Comment		Year	2011
Medium	Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum.	Methods for Passages	Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage	split ratio = 1/5	Race
CO2 Conc.	5%	Tissue Sampling	esophagus
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_8510

Reference
Pubmed id:17935131	Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma and its clinical significance. Ortiz CM,Ito T,Tanaka E,Tsunoda S,Nagayama S,Sakai Y,Higashitsuji H,Fujita J,Shimada Y Int J Cancer. 2008 Jan 15;122(2):325-32
Pubmed id:16951226	The biological role of the low-affinity p75 neurotrophin receptor in esophageal squamous cell carcinoma. Okumura T,Tsunoda S,Mori Y,Ito T,Kikuchi K,Wang TC,Yasumoto S,Shimada Y Clin Cancer Res. 2006 Sep 1;12(17):5096-103
Pubmed id:17671398	ABCG2 expression is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma. Tsunoda S,Okumura T,Ito T,Kondo K,Ortiz C,Tanaka E,Watanabe G,Itami A,Sakai Y,Shimada Y Oncology. 2006;71(3-4):251-8
Pubmed id:15814639	Prognostic significance of fascin overexpression in human esophageal squamous cell carcinoma. Hashimoto Y,Ito T,Inoue H,Okumura T,Tanaka E,Tsunoda S,Higashiyama M,Watanabe G,Imamura M,Shimada Y Clin Cancer Res. 2005 Apr 1;11(7):2597-605
Pubmed id:14522885	Identification of ZASC1 encoding a Krüppel-like zinc finger protein as a novel target for 3q26 amplification in esophageal squamous cell carcinomas. Imoto I,Yuki Y,Sonoda I,Ito T,Shimada Y,Imamura M,Inazawa J Cancer Res. 2003 Sep 15;63(18):5691-6
Pubmed id:12538476	Cell culture in esophageal squamous cell carcinoma and the association with molecular markers. Shimada Y,Maeda M,Watanabe G,Yamasaki S,Komoto I,Kaganoi J,Kan T,Hashimoto Y,Imoto I,Inazawa J,Imamura M Clin Cancer Res. 2003 Jan;9(1):243-9
Pubmed id:9033652	Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines. Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860	Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:7913084	Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M Int J Cancer. 1994 Jul 15;58(2):291-7

Movies

Cell No.	JCRB1418	Cell Name	KYSE790
LOT No.	07292011	Lot Specification	distribution
Medium	RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911)	Temperature	37 C
Cell Density at Seeding	2.2-5.1x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/5)
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.3x10^6
Viability at cell freezing (%)	99.9	Antibiotics Used	free
Passage Number	p4*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:11 D13S317:13 D7S820:10,11 D16S539:9,10 VWA:16,18 TH01:9 AM:X TPOX:8 CSF1PO:10
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Cell Banker BLC-1(Nihon Zenyaku Industries)	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB1418	Cell Name	KYSE790
LOT No.	12022016	Lot Specification	distribution
Medium	Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal bovine serum (FBS; Sigma Cat. # 172012).	Temperature	37 C
Cell Density at Seeding	0.6 - 1.7 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	24 - 83 hrs.	Cell Number in Vial (cells/1ml)	2.5 x 10^6
Viability at cell freezing (%)	78.1	Antibiotics Used	free
Passage Number	Unknown (6 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - Ham's F12 + RPMI1640 (1:1 mixture)	CO2 Conc.	5%
Viability immediately after thawing (%)	NT	Additional information

JCRB Cell Bank

JCRB1418 KYSE790

Cell information

LOT Information