JCRB1418 KYSE790

Cell information

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KYSEシリーズ細胞株の民間企業への分譲について

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1418 Cell Name   KYSE790
Profile   Human well differentiated squamous cell carcinoma cell line established from esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   68
Identity   available Tissue for Primary Cancer   esophagus
Case history   well differentiated squamous cell carcinoma, stage 4 Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   T2M1N1 stage 4
Classify   tumor Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2011
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   esophagus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8510
Reference
Pubmed id:17935131 Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma and its clinical significance.
Ortiz CM,Ito T,Tanaka E,Tsunoda S,Nagayama S,Sakai Y,Higashitsuji H,Fujita J,Shimada Y
Int J Cancer. 2008 Jan 15;122(2):325-32
Pubmed id:17671398 ABCG2 expression is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma.
Tsunoda S,Okumura T,Ito T,Kondo K,Ortiz C,Tanaka E,Watanabe G,Itami A,Sakai Y,Shimada Y
Oncology. 2006;71(3-4):251-8
Pubmed id:16951226 The biological role of the low-affinity p75 neurotrophin receptor in esophageal squamous cell carcinoma.
Okumura T,Tsunoda S,Mori Y,Ito T,Kikuchi K,Wang TC,Yasumoto S,Shimada Y
Clin Cancer Res. 2006 Sep 1;12(17):5096-103
Pubmed id:14522885 Identification of ZASC1 encoding a Krüppel-like zinc finger protein as a novel target for 3q26 amplification in esophageal squamous cell carcinomas.
Imoto I,Yuki Y,Sonoda I,Ito T,Shimada Y,Imamura M,Inazawa J
Cancer Res. 2003 Sep 15;63(18):5691-6
Pubmed id:12538476 Cell culture in esophageal squamous cell carcinoma and the association with molecular markers.
Shimada Y,Maeda M,Watanabe G,Yamasaki S,Komoto I,Kaganoi J,Kan T,Hashimoto Y,Imoto I,Inazawa J,Imamura M
Clin Cancer Res. 2003 Jan;9(1):243-9
Pubmed id:9033652 Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860 Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:7913084 Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M
Int J Cancer. 1994 Jul 15;58(2):291-7
Pubmed id:15814639

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LOT Information

Cell No.   JCRB1418 Cell Name   KYSE790
LOT No.   07292011 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.2-5.1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/5)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   99.9 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:13
D7S820:10,11
D16S539:9,10
VWA:16,18
TH01:9
AM:X
TPOX:8
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1418 Cell Name   KYSE790
LOT No.   12022016 Lot Specification   distribution
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.6 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   24 - 83 hrs. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   78.1 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Ham's F12 + RPMI1640 (1:1 mixture) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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