JCRB1424 KYSE2400
Cell information
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Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1424 | Cell Name | KYSE2400 |
|---|---|---|---|
| Profile | Human moderately differentiated squamous cell carcinoma cell line established from esophageal cancer. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 69 |
| Identity | available | Tissue for Primary Cancer | esophagus |
| Case history | moderately differentiated squamous cell carcinoma, stage 3 | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | T4M1N0 stage 3 |
| Classify | tumor | Established by | Shimada, Y. |
| Registered by | Shimada, Y. | Regulation for Distribution | |
| Comment | Year | 2011 | |
| Medium | Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum. | Methods for Passages | Cells harvested after treatment with trypsin and EDTA. |
| Cell Number on Passage | split ratio = 1/5 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | esophagus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:16951226 | The biological role of the low-affinity p75 neurotrophin receptor in esophageal squamous cell carcinoma. Okumura T,Tsunoda S,Mori Y,Ito T,Kikuchi K,Wang TC,Yasumoto S,Shimada Y Clin Cancer Res. 2006 Sep 1;12(17):5096-103 |
| Pubmed id:17179732 | Gli-1 expression is associated with lymph node metastasis and tumor progression in esophageal squamous cell carcinoma. Mori Y,Okumura T,Tsunoda S,Sakai Y,Shimada Y Oncology. 2006;70(5):378-89 |
| Pubmed id:17671398 | ABCG2 expression is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma. Tsunoda S,Okumura T,Ito T,Kondo K,Ortiz C,Tanaka E,Watanabe G,Itami A,Sakai Y,Shimada Y Oncology. 2006;71(3-4):251-8 |
| Pubmed id:15814639 | Prognostic significance of fascin overexpression in human esophageal squamous cell carcinoma. Hashimoto Y,Ito T,Inoue H,Okumura T,Tanaka E,Tsunoda S,Higashiyama M,Watanabe G,Imamura M,Shimada Y Clin Cancer Res. 2005 Apr 1;11(7):2597-605 |
| Pubmed id:11520067 | Gene expression profiling in human esophageal cancers using cDNA microarray. Kan T,Shimada Y,Sato F,Maeda M,Kawabe A,Kaganoi J,Itami A,Yamasaki S,Imamura M Biochem Biophys Res Commun. 2001 Aug 31;286(4):792-801 |
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LOT Information
| Cell No. | JCRB1424 | Cell Name | KYSE2400 |
|---|---|---|---|
| LOT No. | 09142012 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 2.2-3.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTACells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Split ratio=1/5. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.66x10^6 |
| Viability at cell freezing (%) | 97.5 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:13 D7S820:10,12 D16S539:9 VWA:14,16 TH01:9 AM:X TPOX:9,11 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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