JCRB9117 BCL1 Clone CW13.20

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9117 Cell Name   BCL1 Clone CW13.20
Profile   Mouse B cell line, where the differentiation is stimulated by B cell differentiation factors. Other Name   
Animal   mouse Strain   BALB/c
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   spontaneous tumor Metastasis   
Tissue Metastasized    Genetics   murine B cell leukemia, BALB/c
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   murine B cell leukemia, subclone 3B3 of BCL1 clone CW13.20.
Classify   tumor Established by   Brooks,K. & Vitetta,E.
Registered by    Regulation for Distribution   ATCC
Comment    Year   1986
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum Methods for Passages   Cells are treated with 0.25% trypsin-0.02% EDTA and scraped.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   B lymphocyte leukemia

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4120
Reference
Pubmed id:6601774Lymphokine-induced IgM secretion by clones of neoplastic B cells.
Brooks K,Yuan D,Uhr JW,Krammer PH,Vitetta ES
Nature. 1983 Apr 28;302(5911):825-6

Images



LOT Information

Cell No.   JCRB9117 Cell Name   BCL1 Clone CW13.20
LOT No.   07072004 Lot Specification   distribution
Medium   RPMI1640 medim with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   0.5 x 10^5 - 1.0 x 10^6 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   31 hrs Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   labeled as [ BCL CL CW13.20 ] on vial
Cell No.   JCRB9117 Cell Name   BCL1 Clone CW13.20
LOT No.   100595 Lot Specification   distribution
Medium   RPMI1640 with 1mM HEPES,NEAAs,1mM Na-pyruvate,0.055mM 2-mercaptoEtOH & 10% heat inactivated FBS. Temperature   37 C
Cell Density at Seeding   1.9x10^5 cells/sq.cm. Methods for Passages   Rince cells with PBS(-) once & treat cells with 0.25% trypsin-0.02% EDTA then scraping.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.04x10^7
Viability at cell freezing (%)   81.9 Antibiotics Used   free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH and NP examined. Mouse.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9117 Cell Name   BCL1 Clone CW13.20
LOT No.   09162016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.2 - 4.2 x 10^5 cells/mL Methods for Passages   Cells were harvested by Pipetting and diluted to fresh medium. Cell attachment is very weak, and cells will form cell aggregates. Some cells will grow as suspension.
Doubling Time   approx. 54 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   87.7 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   87.7 Additional information   
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