JCRB1431 ASF-4-3L

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)

ASF-4シリーズ線維芽細胞の使用目的の限定について / The limitation of use for ASF-4 series fibroblasts

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1431 Cell Name   ASF-4-3L
Profile   Normal diploid fibroblast with limeted life span. The ASF-4-1, ASF-4-2, ASF-4-3 and the ASF-4-4 lines were established from skins of the same donor at different ages. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   56.9-year-old
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   skin fibroblast, diploid (M)
Life Span   finite Crisis PDL   52.2 PDL
Morphology   fibroblast-like Character   normal fibroblast
Classify   primary Established by   Kaji, K.
Registered by   Kaji, K. Regulation for Distribution   Limited to the research for scenescence / rejuvenation. Developer's approval is required.
Comment    Year   2010
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Subculture every 7 days. Split ratio, 1/4. 0.25% trypsin, 10-15 min.
Cell Number on Passage   1x10^4 cells/cm^2 Race   Japanese
CO2 Conc.   5% Tissue Sampling   skin
Tissue Type   skin

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8497
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LOT Information

Cell No.   JCRB1431 Cell Name   ASF-4-3L
LOT No.   11072011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum(SIGMA027K03911,non HI) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Subculture every 7 days.Cells were harvested after treatment with 0.25%trypsin(10-15min).Split ratio=1/4.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.22x10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,10
D13S317:11,12
D7S820:10,11
D16S539:10,11
VWA:17,18
TH01:8,9
AM:X,Y
TPOX:11
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1431 Cell Name   ASF-4-3L
LOT No.   08282015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   6 - 8 x 10^4 cells/cm^2 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   7.1 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
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