-Detailed Information [JCRB9118]-

JCRB9118 SCC-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB9118	Cell Name	SCC-4
Profile	squamous carcinoma, tongue	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity		Tissue for Primary Cancer	oral
Case history		Metastasis
Tissue Metastasized		Genetics	tongue,squamous cell carcinoma
Life Span		Crisis PDL
Morphology		Character	tongue,squamous cell carcinoma
Classify		Established by	Rheinwalt,J.G.
Registered by		Regulation for Distribution
Comment	Culture medium--A 1/1 mixuture of Ham's F12 and Dulbecco's modified with 0.4ug/ml hydrocortisone, 10%FBS.	Year	1986
Medium		Methods for Passages
Cell Number on Passage		Race
CO2 Conc.	5 %	Tissue Sampling	tongue
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	not tested				Detected RNA Virus	not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1684

Reference
Pubmed id:7214336	Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas. Rheinwald JG,Beckett MA Cancer Res. 1981 May;41(5):1657-63

LOT Information

02252008
082898
03182016

Cell No.	JCRB9118	Cell Name
LOT No.	02252008	Lot Specification	distribution
Medium	1:1 mixture of Ham's F12 and DMEM with 0.4 ug/ml hydrocortisone and 10% fetal bovine serum (FBS lot; GIBCO 484375)	Temperature	37 C
Cell Density at Seeding	2 - 3 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA at 37 C for 5-10 min.
Doubling Time	D.T.= 3-4 days	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	P19	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9118	Cell Name
LOT No.	082898	Lot Specification	distribution
Medium	Ham's F12 : Dulbecco's MEM = 1:1 mixture with 0.4 ug/ml hydrocortisone and 10% fetal calf serum	Temperature	37 C
Cell Density at Seeding	2 x 10^4 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA Grow very slowly
Doubling Time	D.T. > 4 days	Cell Number in Vial (cells/1ml)	6.6 x 10^5
Viability at cell freezing (%)	87.0	Antibiotics Used	free
Passage Number	P16	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9118	Cell Name	SCC-4
LOT No.	03182016	Lot Specification	distribution
Medium	1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 mdeium (DMEM/F12) with 0.4 ug/mL hydrocortisone and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091).	Temperature	37 C
Cell Density at Seeding	1.7 - 4.0 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 61 hrs	Cell Number in Vial (cells/1ml)	8.0 x 10^5
Viability at cell freezing (%)	90	Antibiotics Used	free
Passage Number	P16	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:13 D13S317:11,13 D7S820:9,11 D16S539:12 VWA:15,17 TH01:9.3 AM:X,Y TPOX:8 CSF1PO:11
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	90	Additional information