JCRB1450 BT-474/CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1450 Cell Name   BT-474/CMV-Luc
Profile   Luciferase stably expressing cell line (BT-474; Human breast cancer cell line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   60-year-old
Identity   available Tissue for Primary Cancer   
Case history   ductal carcinoma Metastasis   
Tissue Metastasized    Genetics   pLL3.7-luc transfected. Luciferase stably expressing cell line. Selection marker does not exist.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2011
Medium   80% RPMI 1640 + 20% FBS + 10 ug/ml bovine insulin + 2 mM L-glutamine. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio = 1/2 - 1/3 Race   Caucasian
CO2 Conc.   5% Tissue Sampling   mammary gland; breast
Tissue Type   duct

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4Y08
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LOT Information

Cell No.   JCRB1450 Cell Name   BT-474/CMV-Luc
LOT No.   02062012 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) and 10ug/ml bovine insulin Temperature   37 C
Cell Density at Seeding   1-5x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(1/2-1/3 split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.98x10^6
Viability at cell freezing (%)   96.9 Antibiotics Used   
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11
D7S820:9,12
D16S539:9,11
VWA:15,16
TH01:7
AM:X
TPOX:8
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   NT
Additional information   Mycoplasma elimination by MC-210 treatment
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Cell No.   JCRB1450 Cell Name   BT-474/CMV-Luc
LOT No.   03022018 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) and 10ug/ml bovine insulin Temperature   37 C
Cell Density at Seeding   2.0-4.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   p12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11
D7S820:9,12
D16S539:9,11
VWA:15,16
TH01:7
AM:X
TPOX:8
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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