JCRB1458 HUEhT-1

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1458 Cell Name   HUEhT-1
Profile   Immortalized human umbilical vein endothelial cell line established (HUVEC) by electroporation of pIRES-hTERT-hygr. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   HUEhT-1 was established by electroporation of pIRES-hTERT-hygr.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cell culture in collagen I coated dish.
Classify   transformed Established by   Anno, K.
Registered by   Anno, K. Regulation for Distribution   
Comment    Year   2011
Medium   MCDB131 medium supplemented with 10% fetal bovine serum, 0.03g/L endothelial cell growth supplement (ECGS), 5ug/mL heparin, and 10mM L-glutamine. Methods for Passages   trypsinization with 0.025% trypsin-0.265mM EDTA
Cell Number on Passage   split ratio = 1:4 Race   
CO2 Conc.   5% Tissue Sampling   human umbilical vein
Tissue Type   endothelial

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W51
Reference
Pubmed id:17207773Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells.
Anno K,Hayashi A,Takahashi T,Mitsui Y,Ide T,Tahara H
Biochem Biophys Res Commun. 2007 Feb 23;353(4):926-32

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LOT Information

Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   01282016 Lot Specification   distribution
Medium   MCDB131 medium (including 10 mM L-glutamine) supplemented with 10% heat-inactivated fetal bovine serum (SIGMA12J396), 30 ug/mL endothelial cell growth supplement (ECGS), 5 ug/mL heparin-Na on collagen coated dish. Temperature   37 C
Cell Density at Seeding   0.43-0.70x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.05x10^6
Viability at cell freezing (%)   95.00 Antibiotics Used   free
Passage Number   p7 PDL   74
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5 %
Viability immediately after thawing (%)   NT Additional information   Dish:Collagen coated dish
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   07212015 Lot Specification   distribution
Medium   MCDB131 medium supplemented with 10% heat inactivated fetal bovine serum(SIGMA027K03911),endothelial cell growth supplement(ECGS),heparin and L-glutamine,using collagen coated dish. Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.22x10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   
Passage Number   p8* PDL   77
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   03232015 Lot Specification   distribution
Medium   MCDB131 medium supplemented with 10% fetal bovine serum(SIGMA027K03911), 0.03g/L endothelial cell growth supplement (ECGS), 5ug/mL heparin and 10mM L-glutamine (Collagen coat dish). Temperature   37C
Cell Density at Seeding   0.5-1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA.(Split ratio=1/4)
Doubling Time   NT Cell Number in Vial (cells/1ml)   0.99 x 10^6 cells/mL
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   p8* PDL   74
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   10102013 Lot Specification   distribution
Medium   MCDB131 medium supplemented with 10% fetal bovine serum, 0.03g/L endothelial cell growth supplement (ECGS), 5ug/mL heparin, and 10mM L-glutamine (Collagen coat dish). Temperature   37C
Cell Density at Seeding   split ratio = 1:4 Methods for Passages   trypsinization with 0.025% trypsin-EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2 x 10^6 cells/mL
Viability at cell freezing (%)   99.6% Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   75PDL
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   91.5% Additional information   
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   03032012 Lot Specification   distribution
Medium   MCDB131 medium supplemented with 10% heat inactivated fetal bovine serum(SIGMA027K03911),endothelial cell growth supplement(ECGS),heparin and L-glutamine. Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.03x10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   free
Passage Number   p5* PDL   68
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   10162019 Lot Specification   distribution
Medium   MCDB131 medium (including 10 mM L-glutamine) supplemented with 10% heat-inactivated fetal bovine serum (SIGMA12J396), 30 ug/mL endothelial cell growth supplement (ECGS), 5 ug/mL heparin-Na on collagen coated dish. Temperature   37 C
Cell Density at Seeding   0.8-1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   99.50 Antibiotics Used   free
Passage Number   9* PDL   79
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Dish:Collagen coated dish
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   03302021 Lot Specification   distribution
Medium   MCDB131 medium (including 10 mM L-glutamine) supplemented with 10% heat-inactivated fetal bovine serum (SIGMA12J396), 30 ug/mL endothelial cell growth supplement (ECGS), 5 ug/mL heparin-Na on collagen coated dish. Temperature   37 C
Cell Density at Seeding   0.7-0.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   98.00 Antibiotics Used   free
Passage Number   p9* PDL   79
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94.7 Additional information   Dish:Collagen coated dish
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   01242022 Lot Specification   distribution
Medium   MCDB131 medium (including 10 mM L-glutamine) supplemented with 10% heat-inactivated fetal bovine serum (SIGMA12J396), 30 ug/mL endothelial cell growth supplement (ECGS), 5 ug/mL heparin-Na on collagen coated dish. Temperature   37 C
Cell Density at Seeding   0.8-1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.83x10^6
Viability at cell freezing (%)   99.10 Antibiotics Used   free
Passage Number   p9* PDL   78
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   95.7 Additional information   Dish:Collagen coated dish
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   09142022 Lot Specification   distribution
Medium   MCDB131 medium (including 10 mM L-glutamine) supplemented with 10% heat-inactivated fetal bovine serum (SIGMA12J396), 30 ug/mL endothelial cell growth supplement (ECGS), 5 ug/mL heparin-Na on collagen coated dish. Temperature   37 C
Cell Density at Seeding   0.8-1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   99.20 Antibiotics Used   free
Passage Number   p9* PDL   78
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   86.2 Additional information   Dish:Collagen coated dish
Images
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   07102023 Lot Specification   distribution
Medium   MCDB131 medium (including 10 mM L-glutamine) supplemented with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437), 30 ug/mL endothelial cell growth supplement (ECGS), 5 ug/mL heparin-Na. Collagen-coated dish is required for cultivation. Temperature   37 C
Cell Density at Seeding   1.4 - 6.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA. Split ratio=1/4
Doubling Time   approx. 58 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^5
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   76
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - basal culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   97.4 Additional information   
Cell No.   JCRB1458 Cell Name   HUEhT-1
LOT No.   10182023 Lot Specification   distribution
Medium   MCDB131 medium (including 10 mM L-glutamine) supplemented with 10% heat-inactivated fetal bovine serum (SIGMA12J396), 30 ug/mL endothelial cell growth supplement (ECGS), 5 ug/mL heparin-Na on collagen coated dish. Temperature   37 C
Cell Density at Seeding   0.4-0.6x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025%trypsin-EDTA.Split ratio=1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   100.00 Antibiotics Used   free
Passage Number   p9* PDL   78
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   95.9 Additional information   Dish:Collagen coated dish
Images
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