JCRB1464 KMP5

Cell information

Important Notice(s)

本細胞株の民間企業への分譲について

On the MTA of this cell line with Kyoto University (for profit organizations in Japan)

本細胞株の使用について

Announcement of unavailability of this cell line to overseas researcher outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1464 Cell Name   KMP5
Profile   Human moderately differentiated adenocarcinoma cell line established from liver metastasis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   55-year-old
Identity   available Tissue for Primary Cancer   pancreas
Case history    Metastasis   
Tissue Metastasized    Genetics   moderately differentiated adenocarcinoma, stage 4
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Kato, M.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2011
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   liver metastasis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8493
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LOT Information

Cell No.   JCRB1464 Cell Name   KMP5
LOT No.   04062012 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.50-6.72x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.47x10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11,13
D7S820:8,13
D16S539:9,11
VWA:18
TH01:7
AM:X
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1464 Cell Name   KMP5
LOT No.   06032019 Lot Specification   distribution
Medium   1:1 mixture of RPMI1640 and Ham's F12 with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   99.0
Additional information   
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