JCRB1470 SUm/c

Cell information

Important Notice(s)

本細胞株の民間企業への分譲について

On the MTA of this cell line with Kyoto University (for profit organizations in Japan)

本細胞株の使用について

Announcement of unavailability of this cell line to overseas researcher outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1470 Cell Name   SUm/c
Profile   SUm/c was established from floating in the lymph of the thoracic duct of patients with esophageal squamous cell carcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   esophagus
Case history    Metastasis   Y
Tissue Metastasized   lymph node Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   Company users require MTA from the originator. See important notice.
Comment   Distribution restricted to Japan (domestic use only). Year   2011
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   xenograft
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JH10
Images



LOT Information

Cell No.   JCRB1470 Cell Name   SUm/c
LOT No.   07132012 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.0-8.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.25x10^6
Viability at cell freezing (%)   92.8 Antibiotics Used   
Passage Number   p23* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8
D7S820:10,12
D16S539:9
VWA:18
TH01:6
AM:X,Y
TPOX:11
CSF1PO:13,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
CLOSE