JCRB1505 Ishikawa 3-H-12

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1505 Cell Name   Ishikawa 3-H-12
Profile   Estrogen receptor (ER), progesterone receptor (PR) expressing cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   39-year-old
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Nishida, M.
Registered by   Nishida, M. Regulation for Distribution   
Comment    Year   2012
Medium   MEM with 15% non-heat-inactivated Fetal Bovine Serum (FBS). Methods for Passages   Split 1:8, using 0.1% trypsin 0.02% EDTA.
Cell Number on Passage   0.5-4x100,000 cells/cm2 Race   
CO2 Conc.   5% Tissue Sampling   Endometrium
Tissue Type   Cancer on adenocarcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_D199
Reference
Pubmed id:27107655Changes of heterogeneous cell populations in the Ishikawa cell line during long-term culture: Proposal for an in vitro clonal evolution model of tumor cells.
Kasai F,Hirayama N,Ozawa M,Iemura M,Kohara A
Genomics. 2016 Jun;107(6):259-66
Pubmed id:9183638Establishment of eighteen clones of Ishikawa cells.
Nishida M,Kasahara K,Oki A,Satoh T,Arai Y,Kubo T
Hum Cell. 1996 Jun;9(2):109-16

Images



LOT Information

Cell No.   JCRB1505 Cell Name   Ishikawa 3-H-12
LOT No.   12112012 Lot Specification   distribution
Medium   MEM(GIBCO) with 15% fetal bovine serum(SIGMA027K03911,non HI) Temperature   37 C
Cell Density at Seeding   0.5-4.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA,Split ratio=1/8
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.13x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9,12,13
D7S820:9,10
D16S539:9
VWA:14,17
TH01:9,10
AM:X
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1505 Cell Name   Ishikawa 3-H-12
LOT No.   04192018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.7 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   88 Additional information   
Cell No.   JCRB1505 Cell Name   Ishikawa 3-H-12
LOT No.   04152022 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Biowest Cat. # S1820-500 Temperature   37 C
Cell Density at Seeding   0.6 - 2.6 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,11
D13S317:9,12,13
D7S820:9,10
D16S539:9
VWA:14,17
TH01:9,10
AM:X
TPOX:8
CSF1PO:11,12,13
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.3 Additional information   
Images
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